肉苁蓉总苷对阿尔茨海默病模型大鼠的保护作用研究  

Protective Effect of Total Glycosides of Cistanches Herba on Alzheimer’s Disease Rats

作　　者：罗兰[1] 吴小川[2] 高惠静[1] 吕顺忠[1] 王建华[1] 王晓雯[3] 

机构地区：[1]新疆医科大学第一附属医院药学部,乌鲁木齐830054 [2]新疆医科大学基础医学院形态中心,乌鲁木齐830054 [3]新疆医科大学药学院药理教研室,乌鲁木齐830054

出　　处：《中国药房》2013年第23期2122-2125,共4页China Pharmacy

基　　金：新疆维吾尔自治区自然科学基金资助项目(No.200121112)

摘　　要：目的:研究肉苁蓉总苷(GCs)对阿尔茨海默病(AD)模型大鼠的保护作用。方法:大鼠采用脑立体定向技术向海马背侧处注射凝聚态β-淀粉样蛋白(Aβ25-35)以复制大鼠AD模型。实验分为假手术(等容生理盐水)、模型(等容生理盐水)、维生素E(40mg/kg)与GCs高、中、低剂量(160、80、40mg/kg)组。于复制模型前4d开始灌胃给药,每天1次,连续14d。以被动回避性跳台实验和电迷宫实验法检测大鼠学习记忆能力;制备大鼠海马区组织匀浆及脑组织切片,检测大鼠海马区组织乙酰胆碱酯酶(AchE)活性,电子显微镜观察大鼠海马CA1区细胞内Ca2+含量。结果:与假手术组比较,模型组大鼠反应时间显著延长、错误次数显著增多(P<0.01),海马组织AchE活性显著增强(P<0.01),海马CA1区中细胞内Ca2+含量明显增加。与模型组比较,GCs高、中、低剂量组大鼠反应时间显著缩短、错误次数显著减少(P<0.01),海马组织中AchE活性显著减弱(P<0.01或P<0.05),海马CA1区中细胞内Ca2+含量明显降低。结论:GCs能够降低AD模型大鼠海马组织中AchE活性和Ca2+含量,维持脑内乙酰胆碱(Ach)的正常水平并提高学习记忆能力。OBJECTIVE： To investigate the protective effect of total glycosides of Cistanches Herba （GCs） on Alzheimer＇ s dis-ease （AD） rats. METHODS： AD model was induced by injected with condensed Aβ25-35 on the dorsal part of hippocampus by brain stereotactic technique. Model rats were divided into 6 groups, i.e. sham-operated group （constant volume of normal saline）, model group（constant volume of normal saline）, vitamine E group （Vit E, 40 mg/kg） and GCs high-dose, medium-dose and low-dose groups （160, 80, 40 mg/kg）. 4 days before modeling, they were given medicine intragastrically once a day for consecutive 14 days. The learning and memorizing ability of the rats were measured through passive avoidance step-through test and electrical maze test; homogenate and cerebral tissue slice ofhippocampus were prepared; the activity of AchE of the rats was measured; the con-tents of intracellular Ca^2＋ in rat＇ s hippocampus CA1 area were observed under electronic microscope. RESULTS ： Compared with sham-operated group, the erroneous numbers and latency escape were significantly prolonged in model group（P〈0.01 ）, the activity of AchE was increased significantly in hippocampus（P〈0.01）, and the contents of Ca^2＋ in hippocampus CA1 area of rats was in-creased. Compared with model group, latency escape was shortened in GCs low-dose, medium-dose and high-dose groups, and the number of error decreased（P〈0.01）; the activity of AchE was decreased in hippocampus（P〈0.01 or P〈0.05）, and the contents of Ca^2＋ in hippocampus CA1 area of rats was decreased. CONCLUSIONS ： GCs can decrease the activity of AchE and the content of Ca^2＋ decreased Ca^2＋ in hippocampus of AD model rats, and maintain the normal level of Ach and improve the learning and memo-rv caoabilitv of AD rats.

关 键 词：肉苁蓉总苷 阿尔茨海默病 乙酰胆碱酯酶 学习记忆能力 钙离子 

分 类 号：R285[医药卫生—中药学] R742[医药卫生—中医学]