仙珍骨宝对骨质疏松模型大鼠的保护作用研究  

Preventive Effect of Xianzhen Gubao on Osteoporosis Model Rats

作　　者：邹丽宜[1] 许碧莲[1,2] 陈文双[2] 张新乐[1] 吴铁[1] 崔燎[1,2] 

机构地区：[1]广东医学院药理教研室,广东湛江524023 [2]广东医学院广东天然药物研究与开发重点实验室,广东湛江524023

出　　处：《中国药房》2013年第23期2130-2132,共3页China Pharmacy

基　　金：东莞市高等院校科研机构科技计划资助项目(No.2008108101047)

摘　　要：目的:研究仙珍骨宝对骨质疏松模型大鼠的保护作用。方法:通过灌胃醋酸泼尼松(3.5mg/kg)每天1次、连续12周以复制大鼠腰椎骨质疏松模型。实验分为正常对照(等容生理盐水)、模型(等容生理盐水)与仙珍骨宝高、低剂量(0.95、0.475g/kg)组。灌胃给药,每天1次,连续12周。末次给药后取大鼠腰椎骨分别测定骨密度、骨生物力学参数和骨形态计量学参数。结果:与正常对照组比较,模型组大鼠腰椎骨密度和断裂载荷显著下降,骨组织总面积、骨小梁面积、骨小梁周长、骨矿化率和生长板荧光标记间距显著减少(P<0.01或P<0.05)。与模型组比较,仙珍骨宝高剂量组大鼠腰椎骨密度、生物力学参数、骨组织总面积、骨小梁面积、骨小梁周长、骨矿化率和生长板荧光标记间距均显著增加(P<0.01或P<0.05)。结论:仙珍骨宝对6月龄SD大鼠灌胃醋酸泼尼松所致的骨质疏松有较好的预防作用。OBJECTIVE： To study preventive effect of Xianzhen gubao on posteoporosis model rats. METHODS： Bone loss model of lumbar vertebrae was induced by intragastric administration of prednisone （3.5 mg/kg）,All of them were treated intragas-trically once a day for 12 weeks. Model rats were divided into 4 groups, i.e. normal control group （constant volume of normal sa-line）, model group （constant volume of normal saline） and Xianzhen gubao high-dose and low-dose groups （0.95 g/kg, 0.475 g/ kg）. All of them were treated intragastrically once a day for 12 weeks. After last medication, BMD, biomechanical properties and bone histomorphometric parameters of lumbar vertebrae were determined. RESULTS： Compared with normal control group, BMD, biomechanical properties and histomorphometric parameters （TAr, TbAr, TbPm, MAR） and the interlabeled width of growth plate of lumbar vertebrae were significantly decreased in model group （P〈0.05）. Compared with model group, BMD, biomechanical properties, bone histomorphometric parameters and the interlabeled width of growth plate of lumbar vertebrae were increased signifi-cantly in Xianzhen gubao high-dose group （P〈0.05）. CONCLUSIONS： Xianzhen gubao has good preventive effect on predni-sone-induced osteoporosis in 6 month old SD rats.

关 键 词：骨质疏松 泼尼松 仙珍骨宝 骨密度 骨生物力学 骨形态计量学 

分 类 号：R285[医药卫生—中药学] R682[医药卫生—中医学]