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作 者:白雪媛[1] 孙立霞[1] 常桂娟[1] 杨吉平[1] 赵雨[1] 赵大庆[1]
机构地区:[1]长春中医药大学,长春130117
出 处:《中国药房》2013年第23期2155-2158,共4页China Pharmacy
基 金:国家科技重大专项资助课题(No.2011ZX09401-305-02);国家科技支撑计划资助课题(No.2012BAI29B05);吉林省科技发展计划资助项目(No.YYZX201134)
摘 要:目的:建立测定参维软胶囊中总皂苷、3种人参皂苷与总多糖含量的方法。方法:采用紫外分光光度法分别于545和490nm波长处测定参维软胶囊中总皂苷和总多糖的含量;采用高效液相色谱法同时测定人参皂苷Re、Rg1、Rb1的含量:色谱柱为Diamonsil C18(250mm×4.6mm,5μm),流动相为乙腈-水(梯度洗脱),检测波长为203nm,柱温为30℃,流速为1.0ml/min。结果:参维软胶囊中总皂苷的平均含量为0.48mg/粒,总多糖的平均含量为0.40mg/粒,人参皂苷Re、Rg1、Rb1的平均含量分别为16.5、19.0、15.9μg/粒。3种含量测定方法均具有较好的线性(r均>0.9990)和较高的精密度、稳定性、重复性(RSD均<3%),平均加样回收率均>92%,RSD均<3%(n=9或n=6)。结论:该方法简便易行、专属性强、准确可靠,可用于参维软胶囊的质量控制。OBJECTIVE: To establish the method for the content determination of total saponins, 3 kinds of ginsenoside and to-tal polysaccharide in Shenwei soft capsules. METHODS: UV spectrophotometry was adopted to determine the contents of total sapo-nins and total polysaccharide in Shenwei soft capsules at detection wavelength of 545 and 490 nm; HPLC method was used to deter-mine the contents of ginsenoside Re, ginsenoside Rgt and ginsenoside Rbt. Diamonsil C18(250mm×4.6mm,5μm) column was used with mobile phase consisted of acetonitrile-water (grandient elution) at the flow rate of 1.0 ml/min. The detection wavelength was set at 203 nm and column temperature was 30 ℃. RESULTS: The content of total saponins was 0.48 mg/tablet, that of total polysaccharides was 0.40 mg/tablet, and the contents of ginsenoside Re, Rg1 and Rb1 were 16.5 μg/tablet, 19.0 μtg/tablet and 15.9 μg/tablet averagely. All the methods had good linearity(r〉0.999 0), precision, stability and reproducibility (RSD〈3%). Average recoveries were all 〉92% (RSD〈3% ,n=9 or n=6). CONCLUSIONS: The methods are simple, specific, accurate and reliable, and it can be used for the quality control of Shenwei soft capsule.
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