烟草中细胞分裂素诱导表达的大豆GmRAV基因参与不定芽再生  

GmRAV,a Cytokinin-Induced Soybean Gene,Involved in Adventitious Bud Regeneration

作　　者：赵琳[1] 崔兆丰[1] 卢清瑶[1] 李冬梅[1] 吕庆雪[1] 张彦威[1] 谷月娇[1] 宋仙萍[1] 王朋朋[1] 李文滨[1] 

机构地区：[1]大豆生物学教育部重点实验室/东北农业大学大豆研究所,黑龙江哈尔滨150030

出　　处：《作物杂志》2013年第3期32-35,共4页Crops

基　　金：黑龙江省教育厅青年骨干教师项目"大豆GmGAMYB1基因参与赤霉素调控开花信号传导途径的机理研究"(1253G010)

摘　　要：通过实时定量RT-PCR研究表明,大豆GmRAV基因的表达受细胞分裂素强烈诱导。对转GmRAV基因烟草研究发现,该基因在0.05mg/L 6-BA存在时,即可促进外植体的不定芽再生,另外,GmRAV基因在过量表达的烟草植株中明显促进烟草细胞周期蛋白CyclinD编码基因的转录,推测其参与细胞增殖和分化过程。RT-PCR analysis showed that soybean GmRAV gene was strongly induced by cytokinins. Transgenic to- baccos overexpressing soybean GmRAV gene exhibited adventitious bud formation under low concentration of 0. 05mg/L 6-BA compared to control plants, indicating that GmRAV promotion of bud regeneration. Overexpression of soybean GmRAV gene in transgenic tobaccos increased the transcript of tobacco cyclinD gene in the process of cell proliferation and differentiation.

关 键 词：烟草 大豆GmRAV基因 不定芽再生 细胞分裂素诱导 

分 类 号：S565.1[农业科学—作物学]