宁心红杞胶囊含药血清指纹图谱研究  

Study on Serum Fingerprint of Menoprogen

作　　者：汝文文[1] 汪光林[2] 韦敏[1] 吕晔[1] 

机构地区：[1]江苏省中国科学院植物研究所,江苏南京210014 [2]江苏省食品药品检验所,江苏南京210018

出　　处：《中药材》2013年第3期428-432,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(30973927)

摘　　要：目的:准确分析确定宁心红杞胶囊入血成分的数量和存在状态(原型成分或代谢产物),建立宁心红杞胶囊的血清色谱指纹图谱。方法:采用Kromasil C18色谱柱,乙腈-0.1%磷酸流动相系统梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长205 nm,对10个不同批号的宁心红杞胶囊内容物混悬液分别灌饲10只SD大鼠的含药血清进行检测并建立指纹图谱。结果:测定给药后入血成分并建立了HPLC指纹图谱,标出18个共有峰,相似度均在0.95以上,与宁心红杞胶囊成分指纹图谱比较分析可知其中8个为原型成分,10个为代谢成分。结论:本实验首次建立了宁心红杞胶囊的血清色谱指纹图谱,确定了18个宁心红杞胶囊的血中移行成分,明确了其中一个入血成分为金丝桃苷,可反映宁心红杞胶囊口服给药吸收入血药物成分的变化,为其体内药效物质研究奠定基础。Objective：To establish chromatographic fingerprint of components of Menoprogen absorbed into blood by RP-HPLC.Methods： Kromasil C18 column was used,with of 0.1% phosphoric acid and acetonitrile as mobile phase in a gradient elution.The flow rate was 1.0 mL/min,the column temperature was 30 ℃,the detection wavelength was 205 nm.10 SD rats were administered 10 different batches of Menoprogen respectively.Results：The fingerprint of Menoprogen was established.18 common peaks were identified,the similarities were over 0.95.By comparison drug and blank serum wiith contained drug serum,identified eight prototype components aborbed into blood directly and 10 metabolic components.Conclusion：The serum fingerprint of Menoprogen is established for the first time.18 components are identifided in blood,one of them is hyperoside.It can reveal the change of chemical constituents after ingestion,and provide some data on material basis study in vivo for Menoprogen.

关 键 词：宁心红杞胶囊 含药血清 指纹图谱 

分 类 号：R961.1[医药卫生—药理学]