余甘子叶提取物对小鼠免疫功能的影响研究  

Study on the Effect of Extracts from the Leaves of Phyllanthus emblica on Immune Function of Mice

作　　者：钟振国[1] 罗雪菲[2] 黄金兰[3] 崔雯[1] 黄丹[1] 冯颖琴 李彩萍[1] 李曼莉[1] 黄志其[1] 

机构地区：[1]广西中医药大学新药研究开发中心,广西南宁530001 [2]广西中医药大学瑞康医院,广西南宁530001 [3]广西医科大学,广西南宁530021

出　　处：《中药材》2013年第3期441-444,共4页Journal of Chinese Medicinal Materials

基　　金：广西科技学技术厅项目(桂科基0639051;桂科攻0718002-2-1;2010GXNSFD013050)

摘　　要：目的:探讨余甘子叶提取物(PLFs)对正常小鼠免疫功能的影响。方法:选取70只健康昆明小鼠进行PLFs的急性毒性试验。各项实验的健康小鼠随机分为对照组及余甘子提取物高(1.982 g/kg)、中(0.991 g/kg)、低剂量组(0.496 g/kg),10只/组,对照组给予等容积双蒸水灌胃。灌胃1次/d,连续给药7 d。小鼠椎脱臼处死取胸腺、脾脏称量质量,计算小鼠胸腺指数和脾指数;尾静脉注射经印度墨汁,眶后静脉丛采血比色测定廓清指数K及吞噬系数α;各组常规制备脾脏细胞,酶标分析仪测定NK细胞杀靶活性和对T、B淋巴细胞的增殖影响情况;腹腔注射绵羊红细胞,测定对小鼠血清溶血素的影响。结果:余甘子叶提取物的LD50为9.911 g/kg;余甘子叶提取物高、中剂量能显著升高小鼠碳廓清能力(P<0.05)、吞噬指数(P<0.05)和NK细胞活力(P<0.05)。结论:余甘子叶提取物具有促进正常小鼠非特异性免疫功能的作用。Objective：To explore the effect of extracts from the leaves of Phyllanthus emblica（PLFs）on the immune function of mice.Methods：70 Kunming mice were choosed to conduct the acute toxicity test of PLFs.The mice were randomly divided into four groups：PLFs high-dosage group,mid-dosage group,low-dosage group and control group.The high,mid,low-dosage groups were treated with PLFs 1.982,0.991 and 0.496 g/kg respectively per day.The same volume of double distilled water was given to the control group.All by intragastric administration for 7 d.The animals were killed and indexes of thymus and spleen were calculated.The expurgation index K and phagocyte index α were detected after the mice being injected with a dilute India ink through caudal vein.In addition,prepared spleen cells conventionally,the activity of Natural Killer cells was measured and the proliferation of T and B cells were detected.The effect of the extracts on serum hemolysin was detected after the SRBC was injected into the enterocoelia.Results：The LD50 of PLFs was 9.911 g/kg.Compared with the control group,the indexes of thymus and spleen in the treatment groups had no markedly difference（P0.05）.The high-and mid-dosage groups could obviously improve the expurgation index K（P0.05）,phagocyte index α（P0.05） and NK cell activity（P0.05）.Conclusion：The extracts from Phyllanthus emblica leaves can promote nonspecific immunity immune function in mice.

关 键 词：余甘子叶提取物 免疫功能 

分 类 号：R285.5[医药卫生—中药学]