柴胡皂苷脂质体的制备工艺及处方优化研究  

Study on Preparation and Optimization of Saikoside Liposomes

作　　者：关延彬[1] 田雨冬[2] 贾永艳[1] 田效志[1] 

机构地区：[1]河南中医学院药学院,河南郑州450046 [2]郑州大学第一附属医院泌尿外科,河南郑州450052

出　　处：《中药材》2013年第3期468-471,共4页Journal of Chinese Medicinal Materials

基　　金：河南中医学院博士科研基金(BSJJ2011-04)

摘　　要：目的:通过优化手段筛选脂质体最佳处方工艺,制备柴胡皂苷脂质体,并对其进行质量评价。方法:采用薄膜分散法、乙醚注入法、逆向蒸发、pH梯度等方法制备柴胡皂苷脂质体,采用大孔吸附树脂测定包封率,优选最佳制备方法。并以包封率为考察指标,采用二因素五水平的星点设计-效应面法优化柴胡皂苷脂质体的处方和工艺。结果:用最优处方制得的脂质体外观好,包封率达到60%以上,平均粒径为110 nm。结论:实验制备的柴胡皂苷脂质体工艺简单可行,为其进一步研究奠定了基础。Objective ：To optimize the prescription and technique for preparing saikoside（SS）liposomes and evaluate its quality.Methods：The preparation methods of liposomes included film dispersion,ether injection,reverse phase evaporation and pH gradients were explored.The encapsulation ratio was determined by macroreticular resin method.The main factors affecting encapsulation ratio were studied by single factor analysis and central composite design.Results ：The appearance of SS liposomes prepared by optimized method was satisfactory.The encapsulation ratio was more than 60% and the verage particle size of SS liposomes was 110 nm.Conclusion： The formulation and preparation process is practical and simple for the preparation of SS liposomes.It is valuable to be further studied.

关 键 词：柴胡皂苷 脂质体 包封率 星点设计 

分 类 号：R286.1[医药卫生—中药学]