基于AFLP方法对不同地理种群棉褐带卷蛾遗传分化的研究  

Analysis of genetic differentiation in different populations of Adoxophyes orana based on AFLP

作　　者：魏晓棠[1] 张成标[1] 张京宣[1] 尼秀媚[1] 胡东青[1] 宋涛[1] 

机构地区：[1]山东出入境检验检疫局,青岛266002

出　　处：《应用昆虫学报》2013年第3期686-692,共7页Chinese Journal of Applied Entomology

基　　金：国家质检总局项目(2010IK275)

摘　　要：利用3对AFLP引物对我国棉褐带卷蛾4个地理种群进行了遗传分化研究。共扩增得到224个DNA条带，其中多态条带195个；遗传多样性分析表明各群体均具有较高的遗传多样性水平，Nei遗传多样性指数为0.1425~0.1578，Shannon多样性指数为0.2137~0.2383。群体间的遗传分化系数（Gst）和基因流（Nm）分析结果表明，北方的苹小卷叶蛾与南方茶小卷叶蛾之间产生了明显的遗传分化，而北方的苹小卷叶蛾不同种群间则存在着大量的基因交流，遗传分化程度很弱。AMOVA分析结果显示66.81%的变异来源于种群内。UMAGA聚类分析表明，北京种群、陕西种群和山东种群间遗传距离较小，聚在一起为一分支，福建的茶小卷蛾单独为一分支。推测棉褐带卷蛾种群间的遗传分化与寄主有关联，与地理距离不相关。The AFLP technique was used to analyze the genetic differentiation among 4 populations of Adoxophyes orana by using 3 pairs of primers, and 224 DNA fragments were generated while 195 of 224 DNA fragments were polymorphic. The genetic diversity analysis of the populations showed high level of genetic variation among 4 populations, and the Shannon’s genetic diversity indexes varied from 0.2137 to 0.2383. Nei genetic diversity indexes were from 0.1425 to 0.1578. Remarkable genetic differentiation indicated by genetic differentiation coefficient （Gst） and gene flow （Nm） analysis among 4 populations, was detected between the Summer fruit tortrix populations in the North and the Smaller tea tortrix populations in the South. There was a relatively strong level of gene flow and a low extent of genetic differentiation among the three Summer fruit tortrix populations in the North. The result of AMOVA analysis indicated that a high percentage of the total genetic variance （66.81%） accounted for the variations among populations. The UPGMA cluster analysis indicated that the genetic distances were closer among Shandong, Beijing and Shanxi populations, and thus the three populations were categorized into one clade, while Fujian population should be categorized into another clade. The results suggested that the genetic distance might be correlated with their host comparability but not related with their geographic distance.

关 键 词：棉褐带卷蛾 AFLP 遗传分化 地理种群 

分 类 号：S436.6[农业科学—农业昆虫与害虫防治]