SSCP技术检测松毛虫赤眼蜂mtDNA编码区SNPs的可行性研究  

Feasibility of using SSCP polymorphism to detect the mtDNA coding region of Trichogramma dentrolimi

作　　者：董辉[1] 谢丽娜[1] 钱海涛[1] 丛斌[1] 王宏侠 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866 [2]抚顺出入境检验检疫局,抚顺113006

出　　处：《应用昆虫学报》2013年第3期874-878,共5页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金(30871674;30971962);国家"十一五"科技支撑计划(2006BAD0806-01);辽宁省高校重点实验室支持计划项目(2008S203);辽宁省教育厅科研项目(L2010488);沈阳农业大学教师科研基金(20091012)

摘　　要：SNPs标记是最具广泛利用潜力的第3代遗传标记,本研究旨在了解SSCP技术检测松毛虫赤眼蜂Trichogramma dentrolimi mtDNA编码区SNPs的灵敏度和精确度,建立大规模筛查SNPs的技术平台。结果表明,运用SSCP技术检测40个松毛虫赤眼蜂样本,单倍型检出率达100%。Cytb和COⅡ基因SNPs的平均密度分别为19SNPs/kb和16 SNPs/kb。SSCP技术操作简单、成本低廉、结果直观、易于分型,适于松毛虫赤眼蜂线粒体编码区未知SNPs的检测,建立了基于SSCP技术检测松毛虫赤眼蜂mtDNA SNPs的技术平台。SNPs are powerful molecular markers. The purpose of this study was to capitalize on the sensitivity and precision of SSCP to detect the Trichogramma dentrolimi mtDNA coding region, and establish an efficient technology to discover SNPs. Our result show that forty T. clentrolimi individuals were detected by SSCP with a haploid detection rate of 100%. The SNPs average density of Cytb and CO II was 19 SNPs/kb and 16 SNPs/kb respectively. SSCP proved relatively easy and cost-effective to use and the results were intuitively and easily detected by genotyping. This method is suitable for detecting unknown SNPs in the mtDNA coding region.

关 键 词：松毛虫赤眼蜂 SNPs标记 SSCP 线粒体DNA 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]