南瓜遗传连锁图谱的构建及粒宽性状的QTL定位  

Construction of Genetic Linkage Map and QTL Analysis for Seed Width in Pumpkin(Cucurbita maxima)

作　　者：谭行之[1] 葛宇[1] 徐文龙[1] 崔崇士[1] 屈淑平[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030

出　　处：《西北植物学报》2013年第4期697-702,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：黑龙江省高校青年学术骨干支持计划(1251G009);公益性行业(农业)科研专项(201303112);东北农业大学博士启动基金(2012RCB30)

摘　　要：以印度南瓜纯系大粒材料‘0515-1’和小粒材料‘0460-1-1’为亲本,获得193个南瓜F2单株群体,应用AFLP和SSR分子标记技术进行多态性筛选,构建了含84个标记位点的遗传连锁图谱。结果表明,整个图谱包含12个连锁群,全长683.50cM,标记平均间距为8.13cM。采用复合区间定位分析,共检测到控制南瓜籽粒宽度的4个数量性状位点(QTL),分别位于3个连锁群上,各QTL的贡献率在2.87%～29.68%之间。With the aim of molecular marker assisted selection for seed width in pumpkin （Cucurbita maxi ma）, 193 progenies of F2 generation derived from a cross between the large seed variety ＇05151 and the small seed variety ＇046011＇ in the present study were screened using AFLP and SSR molecular markers for their polymorphic sites, and a genetic linkage map containing 84 polymorphic loci was constructed for i dentifying seed width trait. The genetic linkage map consisted of 12 linkage groups, and was, totally, 683.50 cM in length. The average distance between adjacent loci was 8.13 cM. Further analysis using the compos ite interval mapping method showed that 4 QTL spread over the 3 linkage groups,the percentage of pheno typic variation explained by individual QTL ranged from 2.87 to 29.68 %.

关 键 词：南瓜 AFLP SSR 遗传图谱 粒宽 QTL定位 

分 类 号：Q343[生物学—遗传学]