他克莫司联合罗格列酮对TNF-α诱导的HaCaT细胞增殖分化的影响  

Effects of combined tacrolimus with rosiglitazone on differentiation of TNF-alpha-induced HaCat cells

作　　者：文晓婷[1,2] 刘彦群[1] 魏志平[1] 张翠[1] 

机构地区：[1]徐州医学院附属医院皮肤科,221002 [2]盐城市第一人民医院,江苏盐城224000

出　　处：《中国麻风皮肤病杂志》2013年第5期303-306,共4页China Journal of Leprosy and Skin Diseases

基　　金：中华医学会皮肤性病学分会-安斯泰来制药(中国)有限公司皮肤病学研究基金

摘　　要：目的：评价他克莫司联合罗格列酮对TNF-α诱导的HaCaT细胞体外增殖和分化的影响。方法：他克莫司（10／maol／L）和不同浓度罗格列酮单独及联合作用于TNF-α诱导的HaCaT细胞48h后，用CCK-8法测定细胞增殖，逆转录一聚合酶链反应（RT—PCR）检测增殖分化标志物角蛋白6及外皮蛋白mRNA的表达水平；结果：他克莫司和罗格列酮均可抑制TNF-α诱导的HaCaT细胞体外增殖，下调角蛋白6mRNA表达，上调外皮蛋白mRNA表达，且联合用药组的作用更强。各组细胞生长抑制率：他克莫司10μmaol／L组为（21．71±5．36）％，罗格列酮组10、20、40、80μmol／L组分别为（5．04±7．12）％、（12．56±6．93）％、（21．41±5．95）％和（39．17±8．03）％，联合1-4组分别为（27．60±6．68）％、（38．35±7．24）％、（48．60±8．40）％和（65．21±4．53）％。结论：他克莫司、罗格列酮单独或联合应用均可抑制TNF-α诱导的HaCaT细胞体外增殖，促进其分化。Objective： To determine the effect of combined tacrolimus with rosiglitazone on the proliferation and differentiation of TNF- ainduced HaCat cells. Method： After TNF- alpha - induced HaCaT cells were treated with tacrolimus （ 10 μmol/L） and rosiglitazone （ 10, 40 μmol/L） single or in a combined way for 48 hours, the prolifera- tion of the cells were detected by CCK- 8 assay, and the expression of kerain6 and involucrin mRNA were semi - quantitatively analyzed by reverse transcription polymerase chain reaction （RT- PCR）. Results： After 48 hours, the proliferation inhibition rate of combined group was significantly higher than single groups. Involucrin mRNA expres- sion was significantly up- regulated, keratin6 mRNA expression significantly down- regulated and the combined groups had stronger effect on the expression. Conclusion： Tacrolimns and rosiglitazone can inhibit the proliferation of TNF- alpha- induced HaCaT cells and induce the differentiation in single or combined way.

关 键 词：他克莫司 罗格列酮 HACAT细胞 外皮蛋白 角蛋白6 

分 类 号：R96[医药卫生—药理学]