积雪草苷对瘢痕组织成纤维细胞Ⅰ、Ⅲ胶原和TGF-β1mRNA表达的影响  

Effects of asiaticoside on the expression of Ⅰ,Ⅲ collagen and TGF-β1 mRNA in keloid fibroblasts

作　　者：李珊珊 王玮蓁[2] 曾宪玉[2] 

机构地区：[1]武汉市第五医院,武汉430000 [2]武汉市第一医院

出　　处：《中国麻风皮肤病杂志》2013年第5期310-313,共4页China Journal of Leprosy and Skin Diseases

摘　　要：目的：评价积雪草苷对培养的原代瘢痕组织成纤维细胞（fibroblast，FB）的细胞增殖活性、自分泌Ⅰ、Ⅲ胶原和转化生长因子p1（transforming growth factorp1，TGF-β1）mRNA的影响。方法：体外培养原代FB，加入不同浓度（0．01、0．05、0．1、0．5、1．0mg／mL）积雪草苷，观察瘢痕FB的形态变化；并用M3T评估不同浓度积雪草苷对瘢痕阳增殖的抑制作用；运用RT-PCR检测Ⅰ、Ⅲ型胶原和TGF-β1 mRNA的表达。结果：与对照组相比，积雪草苷浓度在0．1-1．0mg／mL时，瘢痕FB体积缩小、变短，树突状突起回缩，细胞间隙变大，并呈浓度依赖模式。结论：一定药物浓度范围内，积雪草苷可干预瘢痕形成。Objective： To determine the effects of asiaticoside on the expression of autocrine Ⅰ,Ⅲ collagen and transforming growth factor -β1 （TGF-β1 ） mRNA in cultured keloid fibrohlast cell （ KFB ）. Method： After FB was cultured and asiaticoside added in different concentrations （0.01, 0.05, 0.1,0.5 and 1.0 mg/mL）, the morpho- logical changes of FB observed. The inhibitive effect of asiaticoside on the FB proliferation was measured by MTT as- say. The expression of type Ⅰ, and Ⅲ collagen and TGF -β1 mRNA was detected by RT - PCR. Results： Com- pared with control group, FB volume was reduced, shortened, dendritic neurite was retracted and the cells gap be- came larger when the concentration of asiaticoside in 0.1-1 mg/mL added. These effects were in a concentration dependent manner. Conclusion： Within certain concentration, asiaticoside can intervene the formation of keloid.

关 键 词：瘢痕 成纤维细胞 胶原 TGF-Β1 

分 类 号：R285.5[医药卫生—中药学]