C57BL/6小鼠γδT细胞的组织器官分布和功能特点  

Proportion and characteristics of γδT cells in different tissues and organs of C57BL /6 mice

作　　者：黄俊[1] 罗雪平[1] 陈殿慧[1] 方会龙[2] 谢红艳[3] 

机构地区：[1]广州医学院基础医学院病原学与免疫学教研室,广东广州510182 [2]湘南学院病原学与免疫学教研室,湖南郴州423000 [3]广州医学院机能学实验中心,广东广州510182

出　　处：《细胞与分子免疫学杂志》2013年第5期449-452,457,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30901353);湖南省自然科学基金(11JJ3103);广州市科技新星项目(2011J22007)

摘　　要：目的比较C57BL/6小鼠肝脏、肺脏、脾脏和肠系膜淋巴结中γδT细胞占所分离的淋巴细胞及其CD3+T细胞的百分比、表型和功能的特点。方法分离正常C57BL/6小鼠肝脏、肺脏、脾脏和肠系膜淋巴结的淋巴细胞,应用细胞表面分子染色的方法,使用流式细胞仪观察γδT细胞占从不同组织分离的淋巴细胞及CD3+T细胞的百分比及其表型的特点。细胞经PMA和离子霉素刺激后,应用细胞内细胞因子染色的方法,通过流式细胞仪观察γδT细胞产生IFN-γ、IL-4、IL-9和IL-17细胞因子的情况。结果γδT细胞占分离淋巴细胞中的百分含量在肝脏明显高于肺脏、脾脏和肠系膜淋巴结(P<0.05),而γδT细胞在肠系膜淋巴结CD3+T细胞的百分含量要显著的低于其他脏器(P<0.05)。不同组织器官中γδT细胞以CD4-CD8-表型为主,还存在少量CD8+γδT细胞。在肠系膜淋巴结γδT细胞中CD4+细胞的量明显高于其他器官,且明显可见一群CD4+CD8+细胞。不同组织中γδT细胞中IL-17+的细胞量要明显高于IFN-γ+和IL-4+细胞。γδT细胞基本不分泌IL-9。肺脏的γδT细胞分泌细胞因子的能力最强,其IL-17+细胞的量达到(26.6±12.1)%。IFN-γ+细胞的量在肝脏和肺脏中较高,分别为(1.36±0.37)%和(1.6±0.7)%。结论 C57BL/6小鼠肝脏、肺脏、脾脏和肠系膜淋巴结γδT细胞的含量、表型和功能方面存在显著性差异。Objective To compare the percentages of y6T cells in the lymphocytes and CD3 ＋T cells isolated from the livers, lungs, spleen and mesenteric lymph nodes of C57BL/6 mice and the phonotype and function of y6T cells in different tissues or organs. Methods Lymphocytes were isolated from livers, lungs, spleen and mesenteric lymph nodes of normal C57BL/6 mice, respectively. Then, percentages of y6T cells in lymphocytes and CD3 ＋T cells, and phenotypic characteris- tics of y6T cells were examined by flow cytometry. Moreover, IFN-y, IL-4, IL-9 and IL-17 secreted by 7＇6＂1＂ cells were detec- ted by means of intracellular cytokine staining after stimulation with PMA plus ionomycin. Results The percentage of y6T in lymphocyte cells of the livers was significantly higher than that of the lungs, spleen and mesenteric lymph nodes （ P 〈 0.05）, and the percentage in CD3 ~ T cells of the mesenteric lymph nodes was the lowest （ P 〈 0.05）. CD4- CD8 - 7＇6＂1＂ cells were the main subpopulation in these tissues and organs and there was also a small proportion of CD8 ~ y6T cells. The proportion of CD4 ＋ y6T cells in the mesenteric lymph nodes was significantly higher than that in the others （ P 〈 0.0.5）, and a group of CD4 ＋ CD8 ＋ y6T cells existed obviously. The percentage of IL-17 ＋ cells in y6T cells was significantly higher than IFN-y ＊ and IL-4~ cells, and almost no IL-0＋ y6T cells were found. The ability of secreting cytokines of y6T cells in the lungs was the strongest and the percentage of IL-17 ~ 7＇61＂ was （26.6 ＋ 12.1 ） %. In addition, the proportion of IFN-y＋ y6T cells in the livers and lungs were （ 1.36 ＋ 0.37） % and （ 1.6 ＋ 0.7） %, respectively. Conclusion The proportion, phenotype and function of ,（6T cells had significant difference in the livers, lungs, spleen, mesenteric lymph nodes of C57BL/6 mice.

关 键 词：ΓΔT细胞 表型 细胞因子 免疫器官 

分 类 号：R392.11[医药卫生—免疫学] S852.43[医药卫生—基础医学]