外周血CD4^+CXCR5^+滤泡辅助性T细胞与慢性乙型肝炎患者高球蛋白血症相关  

Peripheral blood CD4^+CXCR5^+ follicular helper T cells are related to hyperglobulinemia of patients with chronic hepatitis B

作　　者：马志远[1] 谢玉梅[1] 王宇[1] 马力[1] 何瑜[1] 张颖[1] 连建奇[1] 郭永红[1] 贾战生[1] 

机构地区：[1]第四军医大学唐都医院全军感染病诊疗中心,陕西西安710038

出　　处：《细胞与分子免疫学杂志》2013年第5期515-518,521,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81170389)

摘　　要：目的检测外周血CD4+CXCR5+滤泡辅助性T细胞(Tfh细胞)的频率及其表面标志,分析与慢性乙型肝炎(乙肝)患者高球蛋白血症的关系。方法收集健康人、乙肝患者及乙肝高球蛋白血症患者的外周血,分离血浆及外周血单个核细胞(PBMC),ELISA检测血浆中IL-21、CXCL13和IFN-γ水平,流式细胞术检测PBMC中CD4+CXCR5+Tfh细胞的频率及其表面PD-1、ICOS及CD40L的表达情况。结果乙肝高球蛋白血症患者外周血CD4+CXCR5+Tfh细胞占CD4+T细胞的百分比(22.6±4.7)%明显高于普通慢性乙肝患者(11.9±3.9)%及健康志愿者(6.8±3.9)%,CD4+CXCR5+Tfh细胞上PD-1和CD40L的表达水平升高,血清IL-21及CXCL13水平升高,而IFN-γ水平降低,差异具有统计学意义(P<0.05)。结论外周血CD4+CX-CR5+Tfh细胞与乙肝高球蛋白血症的发病相关。Objective To detect cell frequency and surface markers of peripheral blood CD4 ＋ CXCR5 ＋ follicular helper T cells （Tfh cells） and analyze the correlation between CD4 ~ CXCR5 ＋ Tfh cells and hyperglobulinemia of patients with chronic hepatitis B. Methods We collected blood samples of 20 HBV infected patients with hyperglobulinemia, 10 chronic HBV infec- ted patients and 10 health volunteers and isolated plasma and peripheral blood mononuclear cells （PBMCs）. The percentage of CD4 ＋ CXCR5 ~ Tfh cells and the expressions of PD-1, ICOS and CD40L on CD4 ~ CXCR5 ~ Tfh cells were detected by flow cytometry. The levels of CXCL]3, IFN-y and IL-21 in plasma were measured by ELISA. Results Compared with the per- centage of CD4 ~ CXCR5 ~ Tfh cells in chronic HBV infected patients （ ] 1.9 ＋ 3.9） and health controls （6.8 ＋ 3.9）, it was higher in HBV infected patients with hyperglobulinemia （22.6 ＋ 4.7, P 〈 0.0.5）. And in the hyperglobulinemia patients, the ex- pressions of PD-1 and CD40L on CD4 ＋ CXCR5 ＋ Tfh cells and the levels of CXCL13 and IL-21 in plasma increased, whereas the level of IFN-y significantly declined （ P 〈 0.05）. Conclusion The results suggest that CD4 ＋ CXCR5 ＋ Tfh cells may partici- pate in the pathogenesis of hyperglobulinemia during HBV infection.

关 键 词：乙型肝炎 高球蛋白血症 滤泡辅助性T细胞 白细胞介素-21 

分 类 号：R512.62[医药卫生—内科学] R446.61[医药卫生—临床医学]