丹参联合川芎嗪对肺纤维化大鼠血清、支气管肺泡灌洗液中TNF-α和TGF-β1水平的影响  

Effects of combination of Salvia and Ligustrazine on TNF-α and TGF-β1 in serum and BALF of rats with pulmonary fibrosis

作　　者：黄成亮[1] 李艳艳[2] 范贤明[1] 王文军[1] 湛晓勤[1] 

机构地区：[1]泸州医学院附属医院呼吸内科,四川泸州646000 [2]泸州医学院附属医院眼科,四川泸州646000

出　　处：《细胞与分子免疫学杂志》2013年第7期673-676,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：四川省杰出青年基金(2009-04-395);泸州市科技局资助项目(07070)

摘　　要：目的观察丹参联合川芎嗪腹腔注射对博来霉素(BLM)致肺纤维化大鼠血清、支气管肺泡灌洗液(BALF)中TNF-α、TGF-β1水平的影响。方法将90只健康雄性SD大鼠,随机分为生理盐水(NS)组、BLM组、地塞米松(DXM)组、不同剂量中药联合干预组(即C1组:小剂量、C2组:中剂量、C3组:大剂量)6组。NS组大鼠气管内灌注NS,其余各组大鼠气管内灌注BLM造模,每天给予NS(NS组、BLM组)、DXM(DXM组)或不同剂量中药联合(C1、C2、C3组)腹腔注射,各组分别于气管内灌注药物后第7、14、28天处死大鼠各5只。肺组织HE染色和Masson染色观察肺泡炎和肺纤维化改变;ELISA检测血清和BALF中TNF-α、TGF-β1的水平。结果与NS组相比,BLM组第7天时肺泡炎较明显,第14天时有所减轻,出现肺纤维化,第28天时肺泡炎继续减轻,肺纤维化程度加重;DXM组不同时间点肺泡炎及肺纤维化程度均较BLM组减轻;C1组与BLM组相比,肺纤维化程度没有明显减轻;C2组和C3组大鼠第14天时肺泡炎及肺纤维化程度较BLM组明显减轻;C2组和C3组大鼠第28天时肺纤维化程度较BLM组明显减轻。与NS组比较,各组大鼠血清和BALF中TNF-α、TGF-β1水平均明显增加;与BLM组比较,DXM组和C2组、C3组不同时间点大鼠血清和BALF中TNF-α、TGF-β1水平均降低;与DXM组比较,不同剂量中药联合干预组不同时间点大鼠血清和BALF中TNF-α、TGF-β1表达均降低;以上比较差异均有统计学意义(P<0.05);C2组和C3组不同时间点血清和BALF中TNF-α、TGF-β1水平差异无统计学意义(P>0.05)。各组大鼠血清和BALF中TNF-α、TGF-β1的水平呈高度一致性。结论中、大剂量丹参联合川芎嗪腹腔注射可减轻BLM致肺纤维化大鼠肺泡炎和肺纤维化程度,其机制可能与降低血清和BALF中TNF-α、TGF-β1的表达水平有关。Objective To observe the effects of Salvia combined with Ligustrazine on the TNF-a and TGF-β1 in sera and bronchoalveolar lavage fluid （BALF） of rats with pulmonary fibrosis induced by bleomycin （BLM）. Methods Male SD rats （n =90） were randomly assigned into 6 groups： normal saline （NS） group, BLM group, dexamethasone （DXM） group and three Chinese traditional herbal groups （C1： small dose group, （32： medium dose group, C3： large dose group）. Rats were challenged intratracheally with BLM to establish pulmonary fibrosis rat models, while the NS group with 0.9% NaCI solution. Rats were given intra-peritoneal injections of normal saline （both the NS group and the BLM group）, dexamethasone （the DXM group）, or Salvia combined with Ligustrazine （the β, C2, C3 groups）. At the same time, 5 rats from the 6 groups were sacrificed at day 7, 14 and ？.8 after administration respectively. HE and Masson staining were performed to observe the extent of alveolitis and fibrosis; the levels of TNF-a and TGF-IS] in both sera and BALF were detected by ELISA. Results Compared with NS group, a great deal of inflammatory cell infiltration into the alveolar was observed in BLM group at day 7, the alveolitis was lessened at day 14, while fibrosis was found ; at day 28, the alveolitis became relieved and the fibrosis developed seriously. The extent of alveolitis and pulmonary fibrosis in DXM group was milder than that in BLM group at day ？, 14 and 28 （ P 〈 O. 0.5）. Compared with BLM group, the extent of pulmonary fibrosis in group C1 didn＇t remarkably change （ P 〉 0.05）. The extent of alveolitis and pulmonary fibrosis in group C2 and C3 was significantly milder than that in BLM group at day 14, and the extent of pulmonary fibrosis in group C-＂2 and C3 was remarkably reduced at day 28 （ P 〈 O. 05）. Compared with NS group, the levels of TNF-cc and TGF-15] in both sera and BALF of the other groups were obviously higher （ P 〈 O. 05）. Compared with BLM group, the lev

关 键 词：丹参 川芎嗪 肺纤维化 TNF-Α TGF-Β1 

分 类 号：R563.1[医药卫生—呼吸系统] R285.5[医药卫生—内科学]