辅酶Q10对脑缺血再灌注损伤大鼠海马区Bcl-2的表达增强及Bax和GSK-3β表达的抑制  

Coenzyme Q10 enhances the expression of Bcl-2 and inhibits the expressions of Bax and GSK-3β in the hippocampus of rats exposed to ischemia/reperfusion injury

作　　者：田爽[1] 王迪[1] 李晓东[2] 唐健杰[1,3] 韩光[1,4] 戴永逸[1] 

机构地区：[1]辽宁医学院基础医学院细胞生物学教研室,辽宁锦州121000 [2]解放军第205医院重症医学科,辽宁锦州121000 [3]锦州市第二医院药剂科,辽宁锦州121000 [4]盘锦市中心医院神经内科,辽宁盘锦124000

出　　处：《细胞与分子免疫学杂志》2013年第7期698-701,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：锦州市科学技术计划(11A1E32);国家人社部留学人员科技项目(2011LX007)

摘　　要：目的探索辅酶Q10预处理对脑缺血再灌注损伤大鼠Bcl-2、Bax和糖原合成酶激酶-3β(GSK-3β)表达的影响。方法 36只雄性SD大鼠随机分成假手术组(sham)、缺血再灌注组(I/R)和辅酶Q10预处理组(Q10)。采用线栓法阻断大脑中动脉制备大鼠局灶性脑缺血再灌注模型,HE染色观察组织学变化,免疫组化染色和Western blot法检测海马区Bcl-2、Bax和GSK-3β蛋白表达情况。结果免疫组化染色显示海马区Q10组较I/R组Bcl-2蛋白阳性表达率明显升高,Bax和GSK-3β蛋白阳性表达率明显下降;与I/R组相比,Western blot法结果表明Q10组Bcl-2蛋白表达水平升高,Bax和GSK-3β蛋白表达水平下降,差异均具有统计学意义(P<0.05)。结论辅酶Q10增强缺血再灌注损伤大鼠海马区Bcl-2蛋白表达,抑制Bax和GSK-3β蛋白表达。Objective To investigate the effects of coenzyme QI0 pretreatment on the expressions of Bcl-2, Bax and glycogen synthase kinase-3~ （GSK-3[5） in rats suffering from ischemia/reperfusion injury. Methods Thirty-six adult male SD rats were randomly assigned into 3 groups： sham-operated group （sham）, ischemia/reperfusion group （I/R） and coen- zyme Q10 preconditioning group （QI0）. Focal cerebral ischemia/reperfusion models were established in experimental rats by blocking middle cerebral artery with suture. Histological changes of hippocampal neurons were observed by HE staining. The expressions of Bcl-2, Bax and GSK-3~ were detected by immunohistochemistry and Western blotting. Results Immuno- histochemistry showed that the percentage of Bcl-2 positive cells increased in the hippocampus, while the percentages of Bax and GSK-313 positive cells decreased in Q]0 group compared with I/R group. Western blotting revealed that the expression level of Bcl-2 was higher and the expression levels of Bax and GSK-3[5 were lower in Q10 group than in I/R group. There were significant differences between the two groups （ P 〈 0.05 ）. Conclusion Coenzyme Q10 promoted the expression of Bcl-2 and suppressed the expressions of Bax and GSK-3β in the hippocampus of rats exposed to cerebral ischemia/ reperfusion.

关 键 词：辅酶Q10 脑缺血再灌注 Bcl-2 BAX GSK-3Β 

分 类 号：R392-33[医药卫生—免疫学] R743.31[医药卫生—基础医学]