呼吸道合胞病毒感染细支气管炎患儿外周血树突状细胞的数量及其与临床症状的相关性  

Correlation between the numbers of peripheral blood dendritic cells and the clinical manifestations in children with respiratory syncytial virus bronchiolitis

作　　者：张家祥[1] 翁开枝[1] 梅序桥[2] 张宝忠[1] 郑源海[2] 柯专叶[1] 吴阿阳[2] 

机构地区：[1]福建医科大学附属漳州市医院儿科,福建漳州363000 [2]福建医科大学附属漳州市医院检验科,福建漳州363000

出　　处：《细胞与分子免疫学杂志》2013年第7期748-750,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：2011年福建省医学创新课题(2011-CXB-26)

摘　　要：目的检测呼吸道合胞病毒(RSV)感染细支气管炎患儿外周血髓样树突状细胞(mDC)及浆细胞样树突状细胞(pDC)的数量,并分析它们与疾病严重程度之间的关系。方法采用PCR方法检测鼻咽部分泌物的呼吸道合胞病毒。通过流式细胞仪检测71例RSV感染(轻度、中度和重度)细支气管炎患儿及48例正常婴幼儿外周血mDC及pDC的数量。结果 RSV感染细支气管炎患儿外周血mDC数量明显高于正常婴幼儿(P<0.01),pDC数量明显低于正常婴幼儿(P<0.01)。重度RSV感染细支气管炎患儿入院时外周血mDC、pDC数量均明显低于轻度组,差异有统计学意义(P<0.05)。结论 RSV感染细支气管炎患儿早期外周血mDC明显升高、pDC降低。mDC数量越高,提示其病情越轻;pDC数量越低,提示喘息越重。Objective To detect the quantity of peripheral blood myeloid dendritic cells （mDCs） and plasmacytoid den- dritic cells （pDCs） in children with bronchiolitis infected by respiratory syncytial virus （RSV） and analyze the correlation with the severity of the disease. Methods PCR was used to detect RSV in nasopharyngeal secretions. Flow cytometry was per- formed on the peripheral blood to detect the quantity of mDCs and pDCs in 71 children with bronchiolitis by RSV infection （including mild, moderate and severe infection） and 48 healthy control infants. Results The quantity of peripheral blood mDCs in the children with bronchiolitis by RSV infection was significantly higher than that of healthy controls （ P 〈 0.01 ）, while the number of pDCs was significantly lower than that of healthy controls （ P 〈 0.01 ）. The children with severe bronchiolitis by RSV infection had significantly lower quantity of peripheral blood mDCs and pDCs as compared with the mild group （ P 〈 0.05）. Conclusion The number of mDCs in peripheral blood of the children with RSV bronchiolitis significantly increased at the early stage, and in contrast pDCs were reduced. The increased number of mDCs indicates that the clinical manifestations are slighter, and the decreased number of pDCs suggests more wheezing of the children.

关 键 词：树突状细胞 细支气管炎 呼吸道合胞病毒 

分 类 号：R725.6[医药卫生—儿科] R562.21[医药卫生—临床医学]