OX40/OX40L分子在B6.MRL-Fas^(lpr)/J狼疮小鼠脾脏组织中的表达及意义  

Expression and significance of OX40 and OX40L in the spleen of B6.MRL-Fas^(lpr)/J lupus mice

作　　者：马钰[1] 沈宇[2] 胡玉敏[1] 王勤[1] 张学光[1] 

机构地区：[1]苏州大学医学生物技术研究所,苏州215007 [2]苏州大学附属第一医院135实验室,苏州215006

出　　处：《现代免疫学》2013年第3期184-188,共5页Current Immunology

基　　金：国家自然科学基金(81072451)

摘　　要：为了探讨OX40和OX40L分子在B6.MRL-Faslpr/J(简称B6.Fas)狼疮小鼠淋巴细胞上的表达及其在系统性红斑狼疮(systemic lupus erythematosus,SLE)发病机制中可能的作用,本课题采用流式细胞仪检测技术对小鼠脾脏T细胞上OX40和B细胞上OX40L的表达水平进行分析并采用Albustix试纸法检测小鼠24h尿蛋白量。结果显示,20周龄B6.Fas狼疮小鼠伴有异常升高的蛋白尿(++～+++)、28周龄B6.Fas狼疮小鼠蛋白尿含量进一步升高为++++,12周龄B6.Fas狼疮小鼠及正常小鼠均为≦+。与正常对照小鼠相比,12周龄B6.Fas狼疮小鼠T、B细胞上OX40/OX40L的表达无显著差异(P>0.05);20周、28周龄B6.Fas狼疮小鼠CD4+T细胞上OX40表达水平以及B细胞上OX40L表达水平明显升高(P<0.05),而CD8+T细胞上OX40表达水平无显著差异(P>0.05);并且28周龄B6.Fas狼疮小鼠T、B细胞上OX40/OX40L的表达水平高于20周龄小鼠(P<0.05)。这表明伴随蛋白尿量的增加,狼疮小鼠脾脏T细胞和B细胞上OX40和OX40L分子表达异常升高,提示OX40/OX40L信号可能与狼疮的发病以及病情发展有一定的相关性。We used B6. MRL-Fas^lpr/J （B6. Eas） mouse model of systemic lupus erythematosus （SLE） to investigate the expression of OX40 and OX40L molecules in spleen T lymphocytes and B lymphocytes and to explore their possible roles in the pathogenesis of SLE, OX40 and OX40L expression on T cells and B cells were detected by flow cytometry. Proteinuria was evaluated by Albustix dipstick. The results showed that, compared with the control group, while both the levels of OX40 and OX40L on T cells and B cells in 12 weeks old lupus mice had no significant change（P〉0.05）, but those on CD19~ B cells in 20 weeks old and 28 weeks old lupus mice were significantly upregulated（P〈0.05）, OX40 levels on CD8＋ T cells had no significant change （P〉0.05）. The expression of OX40 and OX40L in the 28 weeks old lupus mice were higher than those in the 20 weeks old ones （P〈0.05）. In the lupus group, protein concentration in the uria was abnormally elevated with age and no protemurla was found in the normal controls. OX40 and OX40L were abnormally expressed on CD4＋ T cells and CD19＋ B cells from the spleen of lupus mice with proteinuria, and the expression levels were increased in correlation with increased proteinuria, suggesting that OX40/OX40L signal may play a critical role in the development of SLE.

关 键 词：B6 MRL-Fas^lpr J狼疮小鼠 协同刺激分子 OX40 OX40L 

分 类 号：R392.11[医药卫生—免疫学]