三丁基锡对小鼠脾、肝和脑组织蛋白磷酸酶2A活性的影响  

The effects of tributyltin on PP2A activity in mouse spleens, livers and brains

作　　者：张亚莉[1] 孙玉涛[1] 翁登坡[1] 徐立红[1] 

机构地区：[1]浙江大学医学院生物化学与遗传学系,浙江杭州310058

出　　处：《癌变．畸变．突变》2013年第3期194-197,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金项目(20777067)

摘　　要：目的:研究三丁基锡(tributyltin,TBT)对小鼠脾、肝和脑组织蛋白磷酸酶2A(protein phosphatase2A,PP2A)活性的影响。方法:将不同浓度TBT(0、10、20、60mg/kg),分别以灌胃的方式对小鼠染毒24和96h,检测脾、肝和脑组织PP2A酶活性的改变。结果:TBT染毒24和96h,小鼠脾脏PP2A酶活性在20和60mg/kg剂量组明显下降,与对照组相比差异均具有统计学意义(P<0.01);肝脏PP2A酶活性仅在60mg/kg时,较对照组明显被抑制(<0.05);而脑PP2A酶活性在各浓度组与对照组相比均未发生明显改变(P>0.05)。结论:PP2A酶活性的降低可能是TBT产生免疫和肝毒性效应的重要分子机制。OBJECTIVE： To investigate the effects of tributyltin（TBT） on the activity of protein phosphatase 2A（PP2A） in the mouse spleens,livers and brains. METHODS：Mice were orally dosed with 0,10,20 and 60 mg/kg of body weight TBT for 24 h and 96 h,and the activity of PP2A was assessed in the mouse spleens,livers,and brains. RESULTS：PP2A activity in the spleens was obviously inhibited in 20 and 60 mg/kg groups treated for 24 h and 96 h compared to the control group （P 0.01）. PP2A activity in the livers was significantly inhibited in the highest dose of TBT （60 mg/kg） for 96 h compared to the control group （P 0.05）. With regard to the PP2A activity in the brains,there were no statistical significance differences between the control and treatment groups （P0.05）. CONCLUSION：This study suggests a critical role of PP2A in the TBT immunologic and hepatic toxicity mechanisms.

关 键 词：三丁基锡 蛋白磷酸酶2A 脾 肝 脑 

分 类 号：R994.6[医药卫生—毒理学]