混合血清法检测献血员血清抗弓形虫抗体  

Detecting Mixed Serum of Blood Donors for Antibodies to Toxoplasma gondii by Dot-IGSS

作　　者：刘宜升[1] 付琳琳[1] 陈明[2] 杜文平[1] 史志旭 

机构地区：[1]江苏省徐州医学院寄生虫学教研室,江苏徐州221002 [2]江苏省徐州医学院传染病学教研室,江苏徐州221002 [3]江苏省徐州市中心血站,江苏徐州221000

出　　处：《地方病通报》2000年第2期24-26,共3页Endemic Diseases Bulletin

基　　金：江苏省教委自然科学基金资助项目!(JW970049)

摘　　要：将徐州市区义务献血员血清 6人一组混合 ,用斑点免疫金银染色法 ( Dot- IGSS)进行检测 ,同时对所有血清用同样方法逐份进行检测 ,以研究混合血清法筛检抗弓形虫抗体的可能性。检测结果显示 ,混合血清法的敏感性为 92 .31% ,特异性为 99.96 % ,混合血清法与逐份血清法的一致率为 99.73% ,Kappa值为 0 .947( P<0 .0 1)。以全国弓形虫平均感染率( 4.86 % )进行定量效益分析 ,用混合血清法筛检献血员 ,每百人可减少检测次数 5 7次 。To prevent blood transfusion toxoplasmosis, the sera of blood donors, per six of which were mixed as a group, were detected by dotimmunogolddilver staining (DotIGSS) and every single serum specimen was detected simultaneously. The results showed that the sensitivity and specificity of mixed serum method were 92.31% and 99.96% respectively. The consistent rate between the two methods was 99.73% and Kappa value was 0.947(P<0.01). According to mean infective rate (4.86%) of Toxoplasma gondii in China, the quantitative analysis of profit indicated that only 43 times be deteted per one hundred sera specimens if when serum pooling method was carried out, while 100 times must be detected per one hundred serum specimens if single serum method was used.

关 键 词：混合血清法 弓形虫 献血员 Dot-IGSS 

分 类 号：R531.8[医药卫生—内科学]