鹌鹑PGCs的传代培养及鉴定  

Subculture and identification of quail primordial germ cells

作　　者：李玉芳[1,2] 洪志勇[1] 李明[1] 张兴峰[1] 林秀娇[1] 李鸿翔[1] 庄益芬[1] 张文昌[1] 

机构地区：[1]福建农林大学动物科学学院,福州350002 [2]黄淮学院,河南驻马店463000

出　　处：《福建畜牧兽医》2013年第3期5-8,共4页Fujian Journal of Animal Husbandry and Veterinary medicine

基　　金：福建省自然科学基金项目(2007J0042)资助

摘　　要：试验采用鸡胚胎制作的成纤维细胞饲养层培养细胞,成功实现鹌鹑原始生殖细胞(PGCs)的传代培养;并通过对比试验,验证了生长因子LIF、SCF、bFGF能够显著提高PGCs存活率和抑制其分化。In this experiment, PGCs of quails were cultured and subcultured successfully in chicken fibroblast feed layer. And contrast test showed that the growth factor （ LIF,SCF,bFGF）can significantly improve the survival rate of PGCs and inhibit their differentiation.

关 键 词：鹌鹑 PGCS 传代 生长因子 

分 类 号：S839[农业科学—畜牧学]