急慢性HBV感染者外周血白细胞IL-6-572位点基因多态性分析  

Single nucleotide polymorphism of interleukin-6 in peripheral blood leukocytes in patients with hepatitis B viral infection

作　　者：高宝霞[1] 高华[2] 张国顺[3] 冯福民[4] 

机构地区：[1]开滦总医院感染性疾病科,河北省唐山市063000 [2]唐山市人民医院检验科 [3]河北联合大学附属医院消化内科 [4]河北联合大学公共卫生学院

出　　处：《实用肝脏病杂志》2013年第3期226-228,共3页Journal of Practical Hepatology

基　　金：河北省卫生厅医学科学研究重点课题(20100128);唐山市科技局科学技术研究与发展指导计划项目(121302019a)

摘　　要：目的探讨成年人群IL-6基因启动子区-597G/A和-572C/G位点多态性与HBV感染的关系。方法采用聚合酶链反应-限制性片断长度多态性法检测179例HBV感染者(包括118例慢性乙型肝炎和61例急性乙型肝炎)和120例健康对照人群外周血IL-6基因启动子区-597G/A和-572C/G位点基因分型。结果本研究在-597位点仅发现GG基因型,未发现GA或AA基因型,而-572位点发现CC、CG和GG基因型;对179例HBV感染者和120例健康对照人群检测发现,IL-6-572 GG基因型频率分别为15.1%和19.1%(P>0.05),而118例慢性乙型肝炎患者GG基因型(18.7%)明显高于61例急性乙型肝炎(8.2%)和健康人(11.7%),差异有统计学意义(P<0.05);在HBV感染患者G等位基因频率(47.9%)显著高于健康对照组(35.2%),但差异无统计学意义(P>0.05),而慢性乙型肝炎患者G等位基因频率(37.7%)明显高于急性乙型肝炎(22.1%)和健康对照组(26.7%),差异有统计学意义(P<0.05);多因素非条件Lo-gistic回归分析显示-572基因多态性与HBV感染后慢性化有关[P=0.038,OR=3.788(1.075～13.333)]。结论IL-6-572C/G基因多态性与HBV感染慢性化有关。Objective To investigate the correlation of single nucleotide polymorphisms of IL-6 to clinical outcomes of patients with hepatitis B viral infection.Methods The polymerase chain reaction-restriction fragment length polymorphism was used to analyze peripheral blood leukocyte IL-6 gene-597G/A and-572C/G polymorphisms in 179 patients including 118 patients with chronic hepatitis B and 61 patients with acute hepatitis B,and 120 healthy persons.Results The GG genotype of IL-6 gene-597,while not GA or AA,was found,and the CC genotype,CG genotype and GG genotype of IL-6 gene-572 were also positively detected in our series;the frequency of GG genotype of IL-6-572 were 15.1% and 19.1%（P0.05）,respectively,in 179 patients with hepatitis B viral infection and in 120 healthy persons,while it was 18.7% in 118 patients with chronic hepatitis B,much higher than 8.2% in 61 patients with acute hepatitis B or 11.7% in 120 healthy controls（P0.05）;the frequency of G allele of-572 C/G was 37.7% in patients with chronic hepatitis B,much higher than 22.1% in patients with acute hepatitis B and 26.7% in healthy controls（P0.05）;the multivariate logistic regression analysis showed that the GG genotype was positively associated with chronicity of hepatitis B viral infection（P=0.038,OR was 3.788 with the range from 1.075 to 13.333）.Conclusion Polymorphism of IL-6 gene-572 C/G plays some roles in the chronicity of hepatitis B viral infection.

关 键 词：乙型肝炎 白细胞介素-6 基因多态性 外周血 

分 类 号：R512.62[医药卫生—内科学]