温度、光强和营养史对羊栖菜无机磷吸收的影响  

Effects of temperature,irradiance level and nutritional history on the uptake of inorganic phosphorus in Hizikia fusiforme

作　　者：徐智广[1] 李美真[1] 孙福新[1] 王志刚[1] 张莹[2] 

机构地区：[1]山东省海水养殖研究所,山东青岛266002 [2]山东省海洋水产研究所,山东烟台264006

出　　处：《南方水产科学》2013年第3期8-13,共6页South China Fisheries Science

基　　金：国家海洋公益性行业科研专项项目(201105008-2;201305005);山东省海洋生态修复重点实验室开放基金(201206)

摘　　要：为了探讨温度、光照和营养史对羊栖菜(Hizikia fusiforme)无机磷吸收的影响,在不同条件下培养藻体,于第0小时、第0.5小时、第1小时、第2小时、第4小时、第6小时和第9小时分别测定培养海水中磷(P)浓度,以此来计算藻体对P的吸收速率。培养条件设置为10℃、20℃和30℃3个温度梯度;0、1 500 lx和6 500 lx 3个光照强度梯度;营养史通过藻体在不同营养盐浓度下培养96 h后获得,其中不同P营养史设0、5μmol·L-1和50μmol·L-13个梯度,不同氮(N)营养史设0、10μmol·L-1和200μmol·L-13个梯度。结果表明,羊栖菜对P的吸收速率在20℃时达到最大值(0.197±0.005)μmol.(h.g)-1,在10℃时最小值为(0.105±0.001)μmol·(h·g)-1;随着培养光强的升高,P吸收速率逐渐递增;随着营养史中P饥饿程度的增加,P的吸收速率递增;而不同N营养史处理的藻体,无氮(0μmol·L-1)和低氮(10μmol·L-1)处理之间无显著差异,高氮营养史(200μmol·L-1)的藻体具有最高的P吸收速率。To investigate the effects of temperature, irradiance level and nutritional history of nitrogen or phosphorus on inorganic phosphorus uptake in Hizikia fusiforme, we cultivated thalli under different conditions , measured the concentration of inorganic phosphorus in media at 0, 0.5th, 1st, 2nd, 4th, 6th and 9th hour, and determined the uptake rate. We set the temperature at 10 ℃, 20 ℃ and 30 ℃, and irradiance levels at 0, 1 500 lx and 6 500 lx in different experiments respectively. Different nutritional histories were obtained by growing thalli at different concentrations of nutrient for 96 h, including 3 levels of phosphorus （0, 5 μmol·L-1and 50 μmol·L-1） and 3 levels of nitrogen（0, 10 μmol·L-1 and 200 μmol·L-1）, respectively. The results show that the uptake rate of inorganic phosphorus reaches maximum at 20 ℃, while the minimum at 10 ℃. With the enhancement of irradiance level, uptake rate increases. Thalli with nutritional history of lower phosphorus concentration show a higher uptake rate of phosphorus, and vice versa. For different nitrogen histories, higher uptake rate is found in alga with higher nitrogen history, and there is no significant difference between 0 and 10 μmol·L-1 nitrogen histories.

关 键 词：羊栖菜 磷 吸收速率 温度 光强 营养史 

分 类 号：S917.3[农业科学—水产科学]