褶纹冠蚌硫氧还蛋白基因克隆及空间结构分析  

Gene cloning and spatial structure analysis of thioredoxin from freshwater mussel Cristaria plicata

作　　者：刘晓妮[1] 胡宝庆[1] 文春根[1,2] 陶志英[1] 刘大伟[1] 

机构地区：[1]南昌大学生物科学系,江西南昌330031 [2]南昌大学生命科学研究院,江西南昌330031

出　　处：《南昌大学学报（理科版）》2013年第2期185-190,共6页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(30960296);江西省教育厅科技基金资助项目(GJJ12024;GJJ10378);江西省科技攻关项目(2004)

摘　　要：运用RACE-PCR方法从褶纹冠蚌血细胞中克隆出硫氧还蛋白(Trx)的cDNA。结果表明褶纹冠蚌TrxcDNA序列全长为1 169bp,其中5′非编码区(UTR)长11bp,3′非编码区长840bp,含有PolyA尾和典型的腺苷酸信号序列AATAAA,开放阅读框长度为318bp,编码105个氨基酸。预测蛋白质分子量为11.6kDa,等电点为5.38,未发现有信号肽。氨基酸序列中含有1个Thioredoxin结构域(T3-K104),区域内包含一个保守的CGPC活化位点。序列同源性比较结果显示褶纹冠蚌Trx与太平洋牡蛎和紫贻贝的氨基酸序列一致性分别为56%、52%。预测的Trx空间结构由5个β折叠和4个α-螺旋组成,α-螺旋包围β折叠形成紧密的球形,这一结构与人的Trx空间结构相似。TheThioredoxin, Trx cDNA from fresh water mussel Cristaria plicata was cloned by rapid ampli- fication of cDNA ends. The results showed that the full-length cDNA sequence of Trx was 1169 bp,consis- ting of a 5＇-terminal untranslated region（UTR）of llbp,a 3＂erminal UTRof 840 bp with a poly（A）tail and typical adenylate signal sequence AATAAA and an open reading frame of 318 bp, which encoded a polypep- tide of 105 amino acids. The predicted molecular weight was 11.6 kDa and the theoretical isoelectric point was 5.38. No signal peptide was found. The amino acid sequence had a Thioredoxin structure domain（T3-- K104） ,which contained a conservative CGPC motif sites. Comparison of sequence homology indicated that the similarity rate of the amino acid sequence with Crassostrea gigas and Mytilus galloprovincialis were 56 and 52 ,respectively. A tight spherical consisting of five t folding, surrounded by four alpha helix, was predicted for the Trx space structure. The structure was similar with that of human Trx.

关 键 词：褶纹冠蚌 硫氧还蛋白 基因克隆 序列 空间结构 

分 类 号：Q959.215[生物学—动物学]