我国猪繁殖与呼吸综合征病毒的分子演化及与疫苗毒株的比较分析  

Molecular evolution comparison analysis between the field and vaccine strains of porcine reproductive and respiratory syndrome virus in China

作　　者：蔡汝健 蒋智勇 刘燕玲 张乐宜 宋长绪 

机构地区：[1]广东省农科院动物卫生研究所/广东省兽医公共卫生公共实验室,广东广州510640

出　　处：《广东农业科学》2013年第8期115-120,共6页Guangdong Agricultural Sciences

基　　金：广东省生猪科技创新体系岗位专家项目(2008A020100020);广东省农业科技攻关项目(2007A020300006);促进科技服务业发展专项(2010A040301010)

摘　　要：为了解我国大陆地区猪繁殖与呼吸综合征病毒(PRRSV)的分子演化特点,为PRRS防控提供参考,对GenBank中收录的60株PRRSV ORF5基因和NSP2基因进行序列分析。根据ORF5基因和NSP2基因构建的进化树分析表明,我国毒株主要分为4大亚群,其中亚群1为主要分支,以2006年分离的JXA1株为代表的HP-PRRSV毒株,我国HP-PRRSV可能起源于CH1-a和HB-1(sh)/2002株,其变异是逐渐演变的过程。目前我国流行的毒株中HP-PRRSV占绝对优势,且与高致病性疫苗株(JAX1和HUN4株等)同源性较高。In order to understand the characteristics of the molecular evolution of porcine reproductive and respiratory syndrome virus （PRRSV） in China and provide a reference for PRRS prevention and control, this study analyzed the ORF5 gene and NSP2 gene sequence of 60 PRRSV strains from GenBank. The phylogenetie tree, based on the the ORF5 genes and NSP＇2 gene, showed that： the PRRSV strains could be divided into four subgroups, subgroup 1 was a main branch, JXA1 strain isolated in 2006 was a representative strain of HP-PRRSV in the subgroup 1, HP-PRRSV might have originated in the CHI-a and HB-1 （sh）/2002 strain, which variation was gradually evolving process. HP-PRRSV was dominant in pandemic strains at present, and the high homology with the highly pathogenic vaccine strains （JAX1 and HUN4 of strains）.

关 键 词：猪繁殖障碍与呼吸综合征病毒 演化 疫苗株 

分 类 号：S852.659.6[农业科学—基础兽医学]