降钙素对人成骨样细胞增殖与细胞膜流动性的影响  

Effect of calcitonin on the proliferation and membrane fluidity of human osteoblast-like cells

作　　者：薛延[1] 袁润英[1] 相东 王红霞[2] 

机构地区：[1]上海铁道大学基础医学院,上海200070 [2]北京市创伤骨科研究所,北京100035

出　　处：《卫生研究》2000年第3期168-169,175,共3页Journal of Hygiene Research

基　　金：国家自然科学基金

摘　　要：为了解降钙素 (CT)对体外培养的人成骨样细胞 OS- 732增殖与细胞膜流动性的影响 ,并与 1,2 5(OH ) 2 D3的作用进行比较 ,在培养液中添加 1IU/ m l CT前后观察 DNA合成速率、细胞增殖、细胞周期和细胞膜流动性的变化。结果表明 CT有明显促进 OS- 732细胞增殖和 DNA合成的作用 ,使 G2 +M期细胞所占的百分比增加 ,而 S期细胞的百分比下降。1,2 5 (OH) 2 D3可使细胞膜流动性增加 ,而 1IU/ m l CT使其下降。结论认为降钙素与 1,2 5 (OH) 2 D3不同 ,它促进人成骨样细胞增殖和降低细胞膜流动性。The effects of calcitonin (CT) on the proliferation and membrane fluidity of human osteoblast\|like cells line OS\|732 were compared with the effect of 1,25(OH) 2D 3. The rate of DNA synthesis, cell proliferation, cell cycle and membrane fluidity of OS\|732 cells were observed. Results showed that 1IU/ml CT stimulated cell proliferation and DNA synthesis. The percentage of cell in the G 2+M phase was increased and the percentage of cell in the synthetic phase was decreased. The membrane fluidity of OS\|732 cells was increased after 0 1μmol/L and 0 01μmol/L 1,25(OH) 2D 3 treatment and was decreased after 1IU/ml CT treatment. It is concluded that calcitonin might increase the cell proliferation and decrease the membrane fluidity.

关 键 词：降钙素 成骨样细胞 细胞增殖 细胞膜流动性 

分 类 号：Q572[生物学—生物化学] R68[医药卫生—骨科学]