微生物驱产出液群落结构与现场生产动态的关系  

Relationship between structures of microbial colony in produced fluids and oil production performance in microbial flooding oil field

作　　者：王慧[1] 宋智勇[1] 郝滨[1] 刘涛[1] 高光军[1] 郭省学[1] 汪卫东[1] 

机构地区：[1]中国石化胜利油田公司采油工艺研究院,山东东营257000

出　　处：《石油学报》2013年第3期535-539,共5页Acta Petrolei Sinica

基　　金：国家高技术研究发展计划(863)项目(2009AA063500)资助

摘　　要：利用末端标记限制性片段长度多态性(T-RFLP)技术,对胜利油田微生物驱油先导试验现场的油藏微生物群落结构进行了跟踪分析。监测数据包括试验区的注入水和4口受效油井。通过多样性指数计算和聚类分析,在时间上和空间上分别比较了油井产出液的微生物群落结构,并且重点分析了其与油井生产动态的关系。结果表明,不同油井、不同时间的群落结构相似性高度一致。这种相似性与油藏代谢产物乙酸变化的一致性说明:微生物群落分析能够很好地反映油藏微生物变化的真实情况;微生物驱油工艺的实施能够促进内源微生物群落多样性的改变,油藏微生物多样性与油井产量在整体上呈负相关。微生物群落结构分析为微生物驱油现场油井受效评价提供了潜在的依据。The petroleum microbial community structure at microbial flooding pilot test field, which located at Shengli Oilfield, was studied by Terminal Restriction Fragment Length Polymorphism （T-RFI.P）. The monitoring data were collected from the injected water and 4 oil wells. Through biodiversities calculation and cluster analysis, the differences of microbial community diversity, the changes of microbial structure over time, and especially the relationship between the diversity and oil production performance were all compared and analyzed, firstly and systematically. The results were as followings： First, microbial structures were analogical time ly, which was accordance with the change of the metabolic product-acetic acid in reservoir, which all showed that microbial communi- ty analysis can represent the changes of petroleum microorganisms authentically. Second, the regulation of the process of microbial flooding test changed the endogenous microbial diversity, conformably and significantly, followed by the decrease of microbial corn munity diversity. And it was negative correlation between petroleum microbial diversity and oil production. The analysis of microbial structure would be an effective and powerful proof to evaluate whether oil well were controlled by the microbial flooding technology.

关 键 词：微生物驱油 油藏 微生物群落多样性 微生物群落结构 油井产量 

分 类 号：TE357.9[石油与天然气工程—油气田开发工程]