怀化侗族高血压人群血淋巴细胞差异蛋白质表达谱的分析  

Preliminary analyses of lymphocyte differentially expressed proteins in Huaihua Dong peoples withhypertension

作　　者：金玲[1] 刘卫武 幸梓[3] 段于峰[1] 李洪亮[1] 

机构地区：[1]怀化医学高等专科学校诊断教研室,湖南省怀化418000 [2]湖南省怀化市通道县人民医院胸外科 [3]湖南省怀化市中医院内科

出　　处：《中国医师杂志》2013年第5期599-603,共5页Journal of Chinese Physician

基　　金：2009年湖南省卫生厅中医药管理局科研课题（2009079）

摘　　要：目的分析怀化侗族高血压患者血淋巴细胞蛋白质谱，寻找高血压患者血淋巴细胞差异蛋白质，研究其在怀化侗族高血压发病机制中的意义。方法应用MALDI-TOF-MS技术检测130例血淋巴细胞标本（侗族高血压50例，汉族高血压40例，侗族正常人群40例）的蛋白质质谱，用Ma-trixScience公司的Mascot对蛋白质质谱数据进行数据库查询比对，并搜索鉴定蛋白。结果三组均获得重复性好的血淋巴细胞蛋白质双向凝胶电泳图谱。其中3个差异表达蛋白点经质谱鉴定。与侗族正常人比较，侗族高血压蛋白质二硫化物异构酶5、热休克蛋白27两个蛋白表达上调（P〈0.01 ）, Chain A, Structure Of Haemoglobin In The Deoxy Quaternary State With Ligand Bound At The Alpha Haems表达差异无统计学意义（P〉0．05）；汉族高血压上述三个蛋白点表达均上调（P〈0．01）。结论蛋白质二硫化物异构酶5、热休克蛋白27在侗族、汉族高血压中高表达，推测这两个蛋白可能与高血压的发生、发展有关。Objective To explore lymphocyte differentially expressed protein profile and its patho- genic significance in Huaihua Dong peoples with hypertension. Methods The lymphocyte protein profiles among 130 cases of blood specimen were detected and identified with matrix-assisted laser desorption ioniza- tion/time of flight mass spectrometry （MALDI-TOF-MS） and Mascot search against protein database. Re- suits The reproducible protein-maps of two-dimensional gel electrophoresis were obtained among three groups. Compared with Dong normal peoples, the protein disulfide isomerase-related protein 5 and heat shock protein 27 showed higher expressions in the Dong peoples with hypertension （ P 〈0. 01 ）, but chain A, structure of haemoglobin in the deoxy quaternary state with ligand bound at the alpha haems is no statisti- cal significance（ P 〉 0. 05 ）. Those three proteins were upregulated in Han peoples with hypertension （ P 〈0. 01 ）. Conclusions The up-regulation of the protein disulfide isomerase-related protein 5 and heat shock protein 27 in the Dong and Han peoples with hypertension suggest that those two proteins might be as- sociated with the occurrence and development of hypertension.

关 键 词：高血压 蛋白质组学 质谱分析法 少数民族 

分 类 号：R544.1[医药卫生—心血管疾病]