miR-152影响NK细胞对JEG-3细胞的杀伤效应  

Overexpression of miR-152 leads to increased NK cell mediated cytolysis in JEG-3 cells

作　　者：朱晓明[1] 韩涛[1] 王华[1] 王鑫[1] 刘成娟[1] 师增增[1] 黄剑磊[1] 

机构地区：[1]第四军医大学唐都医院,陕西西安710038

出　　处：《现代肿瘤医学》2013年第6期1202-1203,共2页Journal of Modern Oncology

基　　金：国家自然科学基金项目(编号:31000660)

摘　　要：目的:研究miR-152分子对NK细胞杀伤JEG-3细胞效应的影响。方法:在滋养细胞肿瘤细胞系JEG-3细胞中分别转染pre-miR-152(实验组),Cy3标记的pre-miRNA-control(阴性对照),空白对照(NC)为未转染的JEG-3细胞。转染48h进行NK细胞的细胞毒测定,观察各组NK细胞对JEG-3细胞的杀伤效应。结果:与对照组相比,实验组NK细胞对JEG-3细胞的平均杀伤率显著增高(P<0.05),且随着效靶比的增高,杀伤率也增大。空白对照组与阴性对照组之间的NK细胞杀伤能力无显著差异(P>0.05)。结论:miR-152可以提高NK细胞对滋养细胞的杀伤作用。Objective：To investigate the effect of miR-152 on NK cell mediated cytolysis in JEG-3 cells.Methods： The JEG-3 cells were transfected with pre-miR-152（test group）,Cy3 dye labeled pre-miRNA-control（negative control）,respectively.JEG-3 cells transfected with nothing were blank control（NC）.48 hours after transfction,cells were used for NK cell cytotoxicity assay to investigate the NK cell mediated cytolysis in JEG-3 cells.Results： After pre-miR-152 transfection,the lyticactivity of NK cells toward JEG-3 cells was dramatically enhanced compared with that in the control groups at each E/T ratio（NK cells to JEG-3 cell ratio,P0.05）,and the cytotoxicity increased proportionately to an increase in the E/T ratio.There was no difference between negative control group and blank control group（P0.05）.Conclusion： This study reveals that overexpression of miR-152 could lead to increased NK cell mediated cytolysis in JEG-3 cells,suggesting that miR-152 may function as an immune system enhancer through up-regulating NK cell mediated cytolysis of host cells.

关 键 词：miR-152 JEG-3细胞 细胞毒 

分 类 号：R714.2[医药卫生—妇产科学]