有机镓对去势大鼠骨代谢及骨量变化的影响  

Effect of organic gallium on bone metabolism and bone mass in ovariectomized rats

作　　者：王佳时[1] 王广斌[1] 付勤[1] 李彬[1] 贺明[1] 

机构地区：[1]中国医科大学附属盛京医院关节骨外科,沈阳110004

出　　处：《中国骨质疏松杂志》2013年第5期426-430,共5页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金项目(81070688)

摘　　要：目的观察有机镓对去势大鼠骨代谢及骨量变化的影响。方法将30只大鼠随机分为假手术组、卵巢切除组和有机镓组。除正常对照组外,其他2组行卵巢切除。12周后假手术组、卵巢切除组给予PBS治疗,有机镓组给予有机镓干预。8周后取大鼠第五腰椎、股骨分别进行Micro-CT测定骨小梁组织结构;组织形态学测骨小梁占总骨量的百分数(BV/TV);生物力学测定股骨颈机械强度检查;生化指标检测比较血清TRAP、ALP、钙、磷。结果Micro-CT测试表明有机镓组平均骨小梁厚度(Tb.Th)、皮质厚度Ct.Th)均明显高于卵巢切除组(P<0.05),BV/TV明显高于卵巢切除组(P<0.05),破骨细胞数显著减少,有机镓组股骨颈平均最大骨折负荷比卵巢切除组高27.4%,血清TRAP、钙、磷减少(P<0.05)。结论有机镓减少骨吸收的同时增加骨形成,对于去势大鼠骨量减少有明显的改善作用。Objective To observe the effect of organic gallium on bone metabolism and bone mass in ovariectomized rats. Methods Thirty rats were randomly divided into sham group, ovarieetomized group, and organic gallium group. Rats in the other 2 groups were ovariectomized except for those in normal control group. After 12 weeks, rats in sham group and ovariectomy group were treated with PBS, while rats in organic gallium group were treated with organic gallium. After the treatment for 8 weeks, the fifth lumbar vertebrae and the femur of rats were collected. The bone trabeeular structure was detected using micro-CT. The percentage of bone trabecular in total bone mass （BV/TV） was detected using histomorphology method. Mechanical strength of the femoral neck was tested using biomechanical test. Biochemical markers including serum TRAP, ALP, calcium, and phosphorus were detected. Results Micro-CT tests showed that the mean trabecular thickness （Tb. Th） and the cortical thickness （ Ct. Th） in organic gallium group were significantly higher than those in OVX group （P 〈 0.05 ）. BV/TV in organic gallium group was also significantly higher than that in OVX group （P 〈 0. 05）. And a significant reduction in the number of osteoclasts was observed in organic gallium group. The average maximum fracture load of the femoral neck in organic gallium group was 27.4% higher than that in OVX group. The serum concentrations of TRAP, calcium, and phosphorus were decreased （ P 〈 0.05）. Conclusion Organic gallium can reduce bone resorption and increase bone formation at the same time. It can significantly ameliorate the bone mass loss in ovariectomized rats.

关 键 词：有机镓 去势大鼠 骨质疏松 骨代谢 骨形态计量学 

分 类 号：R681[医药卫生—骨科学]