仙灵骨葆对卵巢切除小鼠骨质疏松症的影响  

Effect of Xianlinggubao on osteoporosis in ovariectomized mice

作　　者：王培霞[1] 王芳[2] 高利红[1] 庞小芬[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院老年病科,上海200025 [2]上海交通大学医学院医学遗传学教研室

出　　处：《中国骨质疏松杂志》2013年第5期441-445,449,共6页Chinese Journal of Osteoporosis

基　　金：上海市科委基础研究重大项目(09JC1410300)

摘　　要：目的利用卵巢切除骨质疏松症小鼠模型,研究仙灵骨葆抗骨质疏松症的疗效。方法 30只129SV品系雌性小鼠随机分为三组:卵巢假切+安慰剂组(SHAM+NS,0.2 ml.d-1);卵巢切除+安慰剂组(OVX+NS,0.2 ml.d-1),卵巢切除+仙灵骨葆组(OVX+XLGB,500 mg.kg-1.d-1),持续治疗12周后取材,应用Micro-CT检测骨密度(BMD)和骨小梁结构、组织病理切片观察骨形态、三点弯曲试验和压缩试验检测骨生物力学指标。结果 Micro-CT检测股骨BMD,OVX+NS组BMD(498.6±13.0 mg/cm2)较SHAM+NS组(636.5±12.4 mg/cm2)下降22%(P<0.01),OVX+XLGB组BMD(561.0±18.6 mg/cm2)与OVX+NS组相比提高了13%(P<0.05)。Micro-CT检测小鼠腰椎(L2-5)骨小梁结构显示:OVX+NS组骨小梁BV/TV、Tb.Th分别低于SHAM+NS组22%、35%,Tb.Sp高于SHAM+NS组11%,差异有统计学意义(P<0.05)。给予仙灵骨葆治疗后,腰椎BV/TV及Tb.Th分别高于OVX+NS组15%、16%,Tb.Sp低于OVX+NS组9%,具有显著性差异(P<0.05)。三点弯曲和压缩试验检测OVX+NS组股骨和腰椎的最大载荷和最大应力,股骨最大载荷和最大应力较SHAM+NS组显著降低42%、49%,腰椎最大载荷和最大应力显著降低42%、43%(P<0.05)。仙灵骨葆治疗后,股骨和腰椎的最大载荷分别提高了75%和47%,最大应力分别提高了47%和47%,差异有统计学意义(P<0.05)。结论仙灵骨葆能够显著提高卵巢切除引起的骨密度,改善骨微结构破坏,提高骨生物力学参数,表明仙灵骨葆具有良好的抗骨质疏松症疗效。Objective To investigate the therapeutic effect of Xianlinggubao （XLGB） on osteoporosis in ovariectomized （OVX） mouse model. Methods Thirty female 129SV mice underwent OVX （n = 20） or Sham （n = 10） operation. They were then randomly divided into 3 groups： SHAM ＋ normal saline （NS 0.2 ml/d, ic） group, OVX ＋ NS （NS 0.2 ml/d, ic） group, and OVX ＋ XLGB group （500 mg/kg/d）. All mice were consecutively treated for 12 weeks. Then bone mineral density （BMD） and bone trabecula structure were detected using micro-CT. Bone morphology was observed in tissue pathological sections. Bone biomechanical parameters were detected using three-point bending test and compression test. Results Micro-CT data showed that BMD significantly decreased by 22% in OVX ＋ NS group （498.6 ± 13.0 mg/cm2 ） compared to that in SHAM ＋ NS group （636. 5 ± 12.4 mg/cm2 , P 〈0.01 ）. Compared with that in OVX ＋ NS group, BMD in OVX ＋ XLGB group （561.0 ± 18.6 mg/cm2 ） was significantly increased by 13% after 12 - week garage of XLGB （P 〈 0. 05）. Bone volume/tissue volume （BV/TV） and trabecular thickness （Tb. Th） in OVX ＋ NS group significantly decreased, and bone surface/bone volume （BS/BV） and trabecular separation （Tb. Sp） increased when compared to that in sham group （P 〈 0.05）. XLGB could significantly improve BV/TV and Tb. Th in the femur （ ＋ 6% and ＋ 11% ） compared with that in OVX ＋ NS group. Meanwhile BS/BV decreased by 10% and Tb. Sp decreased by 6% （P 〈 0. 05）.According to three-point bending test and compression test, the maximum load and stress of the femur decreased significantly compared to that in SHAM ＋ NS group （ - 42% and - 49% ）. The decrease in the lumbar vertebrae was - 42% and - 43% （ P 〈 0.05）. After XLGB treatment, the maximum load of the femur and the lumbar vertebrae increased by 75% and 47% , and the maximum stress of the femur and the lumbar vertebrae increased by 47% and 47% （P 〈 0.05 ）. Conclusion Tra

关 键 词：仙灵骨葆 骨质疏松症 小鼠模型 卵巢切除 

分 类 号：R285.6[医药卫生—中药学]