PPAR-γ2基因Pro12Ala多态性与2型糖尿病视网膜病变的相关性研究  

Association of Pro12Ala polymorphism of PPAR-γ2 Gene and diabetic retinopathy in type 2 diabetes

作　　者：刘永庆[1] 王丽聪[2] 董静[2] 王晋峰[1] 武圣洁[1] 

机构地区：[1]山西医科大学,中国山西省太原市030001 [2]山西医科大学第一医院眼科,中国山西省太原市030001

出　　处：《国际眼科杂志》2013年第6期1108-1111,共4页International Eye Science

摘　　要：目的:研究过氧化物酶体增殖物激活受体-γ2(PPAR-γ2)基因Pro12Ala多态性与山西地区汉族人群2型糖尿病性视网膜病变(DR)的相关性。方法:选取年龄40～70岁、糖尿病病程10～20a、血压<140/90mmHg且不合并糖尿病肾病的2型糖尿病患者90例,其中无DR组(NDR组)、非增生型DR组(BDR组)和增生型DR组(PDR组)各30例,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术,对所有患者PPAR-γ2基因Pro12Ala多态性位点进行基因分型。结果:该研究人群中存在2种等位基因和3种基因型,其中NDR组中基因型PP,PA,AA分别为40.0%,53.3%和6.7%,BDR组分别为70.0%,30.0%和0%,PDR组分别为76.7%,23.3%和0%;三组间比较,基因型频率(χ2=10.351)和等位基因频率(χ2=10.208)的差异均有统计学意义(P<0.05)。Logistic回归分析结果显示Pro12Ala基因多态性是DR发生的危险因素。结论:PPAR-γ2基因Pro12Ala多态性与山西地区部分汉族人群2型糖尿病视网膜病变有关,且可能是抑制其发生的保护性因子。AIM： To observe the relationship between Prol2Ala polymorphism of peroxisome proliferator activated receptor-γ2 （PPAR-γ2） gene and diabetic retinopathy （DR） with type 2 diabetic mellitus （T2DM） of the Han nationality in Shanxi province. METHODS： Totally 90 patients with T2DM were selected into our research, who were at the age of 40 to 70 years old, diabetic duration from 10 to 20 years, blood pressure 〈140/90mmHg, and without diabetic nephropathy. Thirty persons were respectively divided into non-DR （NDR） group, nonproliferative-DR （BDR） group and proliferative-DR （PDR） group according to the result of ophthalmology examination. The genotypes of Pro12Ala polymorphism of PPAR-γ2 gene were determined by polymerase chain reaction- restriction fragment length polymorphisms （PCR-RFLP） assay in all the patients. RESULTS. PCR results showed that there were 2 alleles and 3 genotypes in the groups. The frequency of genotype PP, PA, AA were 40.0%, 53.3%, 6.7% in NDR group, 70.0%, 30.0%, 0.0% in BDR group, 76.7%, 23.3%, 0% in PDR group, respectively. The allele frequency （X2 = 10. 208） and gene frequency （χ2= 10. 351 ） were statistically significant （P〈0.05） in these groups. Logistic regressionanalysis showed that Prol2Ala polymorphism was a dangerous factor in the incidence of DR. CONCLUSION： The alanine variant of Prol2Ala polymorphism of PPAR-γ2 gene is associated with DR in type 2 diabetes among the Hans in Shanxi area, and the Ala allele might be a protective factor for the development of diabetic retinopathy.

关 键 词：2型糖尿病 糖尿病视网膜病变 过氧化物酶体 增殖物激活受体 基因多态性 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]