微量元素对热应激果蝇存活率及热应激蛋白合成的影响  

THE EFFECTS OF TRACE ELEMENTS ON SURVIVAL RATE AND HSP70 SYNTHESIS OF HEAT-EXPOSED DROSOPHILA MELANOGASTER

作　　者：秦海宏[1] 郭俊生[1] 赵法[1] 李正银[1] 杨林[1] 

机构地区：[1]第二军医大学军队卫生学教研室,上海200433

出　　处：《解放军预防医学杂志》2000年第3期164-166,共3页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：全军医药卫生科研基金!课题 (No 96L0 2 6 0 1)

摘　　要：目的 :探讨微量元素提高热适应能力的作用及可能的机理。方法 :以果蝇为热暴露动物模型 ,在果蝇普通培养基上分别添加 12种微量元素配制成不同组分实验培养基 ,观察各培养基饲养的果蝇在 37± 0 .5℃热暴露 15h后的存活率及热暴露 30min后果蝇热应激蛋白(HSP70 )的含量。结果 :Zn、Fe、Se、Sn、Si和Mo能显著提高热暴露果蝇的存活率 ,、Cr、Mn、Ni、V和Co无明显作用 ,Cu可使果蝇存活率下降 ;、Zn、Fe、Sn、Si、Mo、Cu和Ni能显著增加HSP70含量。结论 :某些微量元素可提高机体热适应能力 ,其作用机理可能与HSP70合成增加有关。To study the effect of some trace elements on heat acclimation ability of heat-stressed Drosophila melanogaster and their probable mechanisms.Methods: To feed Drosophila melanogaster on the culture medium added respectively with tweleve kinds of trace elements, then to examine the survival rate of Drosophila melanogaster after 37±0.5 ℃ heat exposure for 15 h and to determine HSP 70 contents after 37±0.5 ℃ heat exposure for 30 minutes. Results: The results showed that Zn、Fe、Sn、Si、Mo and Se could significantly increase the survival rate of heat-exposed Drosophila melanogaster, Cr、Mn、Ni、V and Co had no obvious effects. Cu decreased the survial rate. Zn、Fe、Sn、Si、Mo、Cu and Ni could remarkably increase the HSP 70 contents of heat-stressed Drosophila melanogaster. Conclusion: These findings suggested that some essential trace elements could promote themoresistance, and the mechanism was propably due to the enhancing of synthesis of HSP70.

关 键 词：微量元素 热应激 存活率 果蝇 热应激蛋白 

分 类 号：Q581[生物学—生物化学] R977.9[医药卫生—药品]