骨髓增生异常综合征细胞周期相关分子表达的初步研究  

Expressions of cell cycle related molecules in myelodysplastic syndrome

作　　者：赵佑山[1] 郭娟[1] 顾树程[1] 费成明[1] 张曦[1] 李晓[1] 常春康[1] 

机构地区：[1]上海交通大学附属第六人民医院血液科,上海200233

出　　处：《诊断学理论与实践》2013年第1期20-23,共4页Journal of Diagnostics Concepts & Practice

基　　金：国家自然科学基金(NNSFC81170463)

摘　　要：目的:研究骨髓增生异常综合征(myelodysplastic syndrome,MDS)患者细胞周期负性调控因子(p21cip1、p27kip1、p57kip2)的表达水平及细胞周期改变。方法:采用流式细胞仪检测MDS患者骨髓单个核细胞(bone marrowmononuclear cell,BMNCs)细胞周期情况,应用实时荧光定量PCR检测其p21cip1、p27kip1、p57kip2 mRNA表达水平。结果:高危MDS组及MDS-AML组(包括10例MDS转白血病患者及8例AML患者)的p21cip1表达水平显著低于正常对照组(P值分别为0.008、0.029),而低危MDS组与正常对照组差异无统计学意义;p27kip1表达水平在各组间差异无统计学意义;低危MDS组、高危MDS组及MDS-AML组p57kip2表达均显著低于正常对照组(P<0.001);高危MDS组及MDS-AML组的S期细胞比例显著高于正常对照组(P<0.05)。G0/G1期及G2/M期细胞比例则在各组间差异无统计学意义,而高危MDS组、MDS-AML组的S期细胞比例显著高于正常对照组。结论:MDS患者BMNCs存在细胞周期负性调控基因表达的异常,与细胞周期的改变相关。Objective To investigate the expression of cyclin-dependent kinase inhibitors （p21cipl, p27kipl, p57kip2） and change of cell cycle in patients with myelodysplastic syndrome （MDS）. Methods Flow cytometric analysis of cell cycle of bone marrow mononuclear cells （BMNCs） was performed. Expressions of p21cipl, p27kipl and p57kip2 mRNA were detected by real-time fluorescence quantitative PCR. Results Expression of p21cipl was reduced in high risk MDS and MDS-AML groups when compared with healthy controls （P=0,008, 0.029 ,respectively）. However, there was no significant difference between low risk MDS group and healthy control. The expression of p27kipl was not different significantly among the four groups, p57kip2 expression was reduced significantly in low risk MDS, high risk MDS and MDS-AML groups when compared with heahhy controls （P〈0.001）. The ratio of S phase cells in high risk MDS and MDS-AML groups was statistically higher than that in healthy controls. No significant differences were found in ratio of G0/G1 and G4/M phase cells. Conclusions The BMNCs from patients with MDS have abnormality in expression of cyclin-dependent kinase inhibitors, which is correlated with the change in cell cycle.

关 键 词：骨髓增生异常综合征 p21cip1 p57rip2 细胞周期 

分 类 号：R551.3[医药卫生—血液循环系统疾病]