PDGF调控子宫内膜异位症在位内膜间质细胞中cofilin-1基因的表达及意义  

Expression of cofilin-1 induced by PDGF in eutopic endometrium of endometriosis

作　　者：穆庆[1] 许艳丽[1] 陈鹏[1] 王丹波[1] 

机构地区：[1]中国医科大学附属盛京医院,辽宁沈阳110004

出　　处：《东南大学学报（医学版）》2013年第3期302-305,共4页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(81070467);辽宁省科学技术计划项目(2009225026)

摘　　要：目的:探讨cofilin-1基因(CFL1)在子宫内膜异位症在位内膜间质细胞(ESC)异常表达与血小板源性生长因子(PDGF)的关系。方法:获取在位子宫内膜组织,分离培养ESC,以不同的时间和不同浓度的PDGF作用ESC,实时定量PCR检测在PDGF刺激前后CFL1的表达。结果:10 ng.ml-1PDGF作用3、6、12 h,CFL1 mRNA表达均增高,其中作用6 h和12 h表达增高有统计学意义(P<0.05);以1、10、100 ng.ml-1的PDGF分别作用ESC 6 h,CFL1 mRNA表达均增高,有统计学意义(P<0.05)。CFL1在ESC中的表达对PDGF有时间依赖性和剂量依赖性。结论:PDGF是子宫内膜异位症在位内膜间质细胞CFL1表达的强诱导剂。Objective： To investigate whether there is a correlation between the expression of cofilin（CFL1） of eutopic endometrium stromal cells （ESC） and PDGF stimulation. Methods： ESCs from twelve patients with advanced ovarian EMs were isolated.In a time-or dose-dependent manner,PDGF was added into culture medium depleted of fetal calf serum.The expression of CFL1 before and after PDGF treatment was detected by real time PCR. Results： PDGF induced the expression of CFL1 in ESC significantly in a time-or dose-dependent manner.ESC were treated with 10 ng·ml-1 PDGF for 0,3,6,and 12h,CFL1 mRNA expression were increased, when compared with non-treated ESC 6 h and 12 h had statistical significance（P〈0.05）.ESCs were treated with 0,1,10,and 100 ng·ml-1 PDGF for 6h,CFL1 mRNA expression were increased with statistical significance （P〈0.05）. Conclusions： PDGF can induce CFL1 expression in ESC.

关 键 词：cofilin-1基因 血小板源性生长因子 子宫内膜异位症 间质细胞 

分 类 号：R711.71[医药卫生—妇产科学]