电感耦合等离子体发射光谱法测定尿铜的评价  

Evaluation of methodology measuring urinary copper using ICP-AES

作　　者：张宪伟[1] 徐静茹[1] 曹波[1] 

机构地区：[1]南京医科大学附属南京儿童医院,江苏南京210008

出　　处：《东南大学学报（医学版）》2013年第3期327-329,共3页Journal of Southeast University(Medical Science Edition)

摘　　要：目的:评价电感耦合等离子体发射光谱法(ICP-AES)测定尿铜。方法:将尿液标本用去离子水稀释5倍,使用JY ULTIMA-2型电感耦合等离子体发射光谱仪,在波长324.754 nm下测定尿铜标本。结果:1个月内对3个浓度水平的尿铜标本各检测分批,每批重复测定3次。批内CV 4.2%～4.6%,批间CV 4.8%～5.0%,总CV 4.6%～4.9%,方法的精密度1.57%～1.66%,回收率97.4%～98.8%,尿铜元素的线性相关系数r=0.999 8。结论:ICP-AES检测尿铜精密度和准确度高,可作为检测尿铜的常规方法。Objective： The evaluation of methodology measuring urinary copper using ICP-AES. Method： The urine specimen were diluted 5 times with deionized wate,followed by detection using the best-working-state JY ULTIMA-2 ICP-AES on the condition of 324.754nm. Result： Three urine specimens were detected in different concentration batched three within one month.Each time,they were detected three times.The range of batch inside was CV 4.2%~4.6%,between batches was CV 4.8%~5.0% and the total CV 4.6%~4.9%.The method degree of precision was 1.57%~1.66%.The standardized recovery was 97.4%~98.8%.The linear correlation coefficient of the urinary copper was r=0.9998. Conclusion： Laboratory tests suggest that the method using ICP-AES to detect urinary copper does not have systemic errores.This method is accurate and reliable which can be applied as a conventional way to detect urinary copper.

关 键 词：电感耦合等离子体发射光谱法 尿铜 方法学评价 

分 类 号：R446.12[医药卫生—诊断学]