去肝交感神经对部分肝切除大鼠肝细胞增殖的影响  

Effect of hepatic sympathetic denervation on hepatocyte proliferation in rats after partial hepatectomy

作　　者：张新胜[1] 赵丹丹[1] 王改平[1] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,河南省新乡市453007

出　　处：《世界华人消化杂志》2013年第15期1381-1386,共6页World Chinese Journal of Digestology

基　　金：河南省基础与前沿技术研究计划基金资助项目;No.082300450540;河南省科技攻关计划基金资助项目;No.112102310652~~

摘　　要：目的:探讨去肝交感神经对肝部分切除大鼠肝细胞增殖的影响.方法:将84只♂SD大鼠随机分为对照组和实验组,每组42只.对照组大鼠行部分肝切除,实验组大鼠去肝交感神经后进行部分肝切除.两组大鼠分别在手术后12、24、36、48、72、120和168h(每个时间点6只大鼠)处死取材,测量各时间点肝/体质量比;用银染法显示肝细胞核仁组织区相关嗜银蛋白(argyrophilic nucleolar organizer region-associated proteins,AgNORs);用免疫组织化学法检测肝细胞增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)的表达.结果:实验组大鼠的肝/体质量比在术后48、168h(分别为0.0311±0.0011和0.0500±0.0056)显著高于对照组(分别为0.0267±0.0008和0.0412±0.0061)(P<0.05),其余各时间点两组间无明显差异;实验组肝细胞AgNORs颗粒平均数在术后36、48、120h(分别为1.82±0.10、2.01±0.14和2.08±0.12)显著低于对照组(分别为2.79±0.08、2.57±0.26和2.46±0.07)(P<0.05);从术后24-168h实验组肝细胞PCNA的表达显著低于对照组(P<0.05).结论:去肝交感神经对肝切除后肝细胞的增殖可能有抑制作用.AIM： To investigate the effect of hepatic sympathetic denervation on hepatocyte proliferation in rats after partial hepatectomy. METHODS： Eighty-four male SD rats were randomly divided into a control group or an experimental group （42 rats for each group）. The control group was subjected to partial hepatectomy （PH）, and the experimental group underwent hepatic sympathetic denervation followed by PH. Rats in both groups were killed at 12, 24, 36, 48, 72, 120 or 168 h （6 rats at each time point） after surgery. The liver to body weight ratio was measured at each time point, and silver staining and immunohistochemical staining were per- formed to detect the expression of argyrophilic nucleolar organizer region-associated proteins （AgNORs） and hepatic proliferation cell nuclear antigen （PCNA） at each time point. RESULTS： At 48 and 168 h after surgery, the liver to body weight ratios in the experimental group increased significantly compared to the control group （0.0311 ± 0.0011 vs 0.0267 ± 0.0008, 0.0500 ± 0.0056 vs 0.0412 ± 0.0061, both P 〈 0.05）. At 36, 48 and 120 h, the average number of hepatocytes containing AgNORs particles was significantly lower in the experimental group than in the control group （1.82 ± 0.10 vs 2.79 ± 0.08, 2.01 ± 0.14 vs 2.57 ± 0.26, 2.08± 0.12 vs 2.46 ± 0.07, all P 〈 0.05）. At 24-168 h, PCNA expression in hepatocytes was significantly lower in the experimental group than in the control group （all P 〈 0.05）. CONCLUSION： Hepatic sympathetic denervation inhibits hepatocyte division and proliferation in rats after partial hepatectomy.

关 键 词：肝再生 去交感神经 部分肝切除 核仁组 织区相关嗜银蛋白 增殖细胞核抗原 

分 类 号：R657.3[医药卫生—外科学]