P.f多期多表位基因真核表达载体的构建和免疫学特性初步研究  

Construction of P.fmultiphase multiepitope gene eukaryotic expression vector and preliminary studyon the immunological characteristics

作　　者：刘文文[1] 王军[2] 刘学武[2] 赵亚[2] 沈燕[2] 姜河[2] 李英辉[2] 

机构地区：[1]第四军医大学航空航天医学系学员五队,陕西西安710032 [2]第四军医大学微生物学与病原生物学教研室,陕西西安710032

出　　处：《中国医药科学》2013年第10期26-27,30,共3页China Medicine And Pharmacy

基　　金：国家自然科学基金项目(30901370)

摘　　要：目的构建恶性疟原虫多期多表位基因的真核表达载体及其免疫学特性的研究。方法在前期研究基础上,将测序正确的恶性疟原虫多期多表位基因克隆入真核表达载体VR1020内并大量制备。以VR1020重组质粒免疫HHD-2小鼠并进行细胞和体液免疫分析。结果成功获得重组真核表达载体,ELISA显示该重组质粒免疫小鼠后获得了较高效价的抗体,而且显示了较好的CTL杀伤活性,同时诱导了高水平的细胞因子。结论本研究为新型疟疾疫苗的研制奠定一定的理论和实践基础。Objective To construct P. falciparum muhiphase muhiepitope gene eukaryotic expression vector and study its immunological characteristics. Methods On the basis of previous research,the gene of the correct AMAMEG was cloned into the eukaryotic expression vector VR1020. After large-scale preparation of plasmid DNA,HHD-2 mice were immunized and cellular and humoral immunity was analyzed. Results The results showed recombinant eukaryotic expression vector was constructed successfully. The high titers of antibody and CTL killing activity were obtained,at the same time high levels of cytokines were induced in this study. Conclusion Our research would lay a certain theoretical and practical basis on new types of malaria vaccine.

关 键 词：恶性疟原虫 疫苗 多表位基因 

分 类 号：R392.1[医药卫生—免疫学]