人IgG1FccDNA的克隆与鉴定  

Cloning and Identification of Human I_gG1Fc cDNA

作　　者：安云庆[1] 柯岩[1] 陈金栋[1] 

机构地区：[1]首都医科大学微生物学和免疫学教研室

出　　处：《首都医科大学学报》2000年第2期93-96,共4页Journal of Capital Medical University

基　　金：国家自然科学基金资助项目 !(395 70 6 6 6 )

摘　　要：根据编码人IgG1Fc(Fcγ1 )的基因序列 ,设计合成 1对与之相匹配的引物。采用RT PCR法 ,从正常人白细胞mRNA中扩增获得预期目的基因Fcγ1 70 0bp基因片段 ,成功构建PUC1 8 Fcγ1 70 0 重组克隆载体 ,酶切、酶谱分析与预期结果相符 ;DNA测序结果与GenBank报道一致。According to the cDNA sequences which encode the human IgG1Fc, a pair of primers were designed; The expected gene (Fcγ1 700 bp ) which encode Fcγ1 was amplified by RT PCR from mRNA extracted from normal person′s leukocyte; PUC18 Fcγ1 700 recombinant cloning vector was successfully constructed, and the enzyme digestion analysis are identical to expected results. Sequences are identical to those in GenBank report.

关 键 词：IgG1Fe(Fcγ1) RT-PCR 克隆 鉴定 

分 类 号：Q78[生物学—分子生物学] R392.1[医药卫生—免疫学]