一种基于氧杂蒽结构的“Turn-Off”型荧光探针研究及对磷酸酯类物质检测  

The Study of a “Turn-Off” Type Fluorescent Probe Based on The Xanthene Structure and Its Detection of the Phosphoric Acid Esters

作　　者：向德成[1] 孟庆华[1] 徐伟健[2] 郑南[1] 谢少艾[1] 郑少瑜[1] 

机构地区：[1]上海交通大学化学化工学院,上海200240 [2]上海交通大学致远学院,上海市200240

出　　处：《信息记录材料》2013年第3期4-8,共5页Information Recording Materials

基　　金：上海市科委纳米专项资助项目(0752nm016)

摘　　要：设计了一种基于氧杂蒽结构的"Turn-Off"型荧光探针(P3),在生理pH条件下对磷酸根(Pi)较好的荧光淬灭性能,在与其它竞争性含氧阴离子(硫酸根、硝酸根、碳酸氢根)以及其它常见阴离子(氟离子、氯离子、溴离子等)对该识别的竞争性影响考察中,亦显示了较好的特异性。从荧光滴定法得到荧光探针(P3)与磷酸根(Pi)形成超分子复合物的摩尔比为1∶1,复合物稳定常数K为5.28*103。通过量子化学计算表明磷酸根与!共轭体系产生了较强的相互作用,并导致P3分子平面上翘,在一定程度上破坏了原先的共面型,磷酸根上氧部位的孤对电子与!共轭体系存在着轨道交叠,即p-!共轭作用。可将此探针应用于磷酸酯类物质的检测。Abstract： A xanthene structure of ＂Turn-Off＂ type fluorescent probe （P3） was de signed, which showed selectively quenching response to phosphate ion （Pi） over other anion ions at the physiological pH condition. The molar ratio of Pi/P3 and stability constant of the supramo lecular complex was obtained as 1 ： 1 and 5.28 ＊ 10^3 , respectively from the fluorescence titration method. The quantum chemical calculation showed that phosphate ion and conjugate system of P3 have a strong interaction, and lead to the molecular plane of P3 molecule upwards, to a certain extent, destroyed the original coplanarity. There electrons on phosphate and part of the conjugate tion. This probe could be applied in detection of t was some orbital overlap between the lone pair system （HOMO） which meant p--p conjuga- he phosphoric acid esters.

关 键 词：荧光探针 氧杂蒽 淬灭 共轭 

分 类 号：TQ31[化学工程—高聚物工业]