江苏口岸常见鼠形动物Cytb基因分析  

Analysis of the common rat-shaped animals' Cytb gene at ports in Jiangsu

作　　者：吴炳耀[1] 叶松[1] 刘国礼[1] 孙立新[2] 

机构地区：[1]安徽理工大学医学院,安徽淮南232001 [2]江苏出入境检验检疫局医学媒介生物监测实验室,江苏南京210001

出　　处：《中华卫生杀虫药械》2013年第3期203-205,208,共4页Chinese Journal of Hygienic Insecticides and Equipments

基　　金：国家质检总局科研基金项目(编号:2009IK220)

摘　　要：目的通过基因分析,建立江苏口岸常见鼠形动物Cytb基因数据库和进化树,为口岸常见鼠形动物进行分子鉴定奠定基础。方法通过基因扩增、构建质粒载体、测序等方法,建立江苏口岸常见鼠形动物Cytb基因数据库。结果获得了褐家鼠、黄胸鼠、小家鼠、黑线姬鼠和大麝鼩等5种鼠形动物全长Cytb基因,并建立了5种鼠形动物Cytb基因进化树。结论建立了江苏口岸常见鼠形动物Cytb基因数据库和进化树,为实现部分鼠形动物的分类鉴定奠定了基础。Objective In order to lay the foundation for molecular space identification through genetic analysis, establish the Cytb gene database of the common rat-shaped animals in Jiangsu and create a phylogenetie tree. Methods By gene amplification, construction of plasmid vector, sequencing,creating the Cytb gene database of the common rat-shaped animals in Jiangsu. Results We obtain the full-length Cytb gene of Rattus norvegicus, Rattus tanezumi,Mus museulus,Apodemus agrarius and Crocidura lasiura,and ereat a rat-shape animals＇ Cytb gene phyloge- netie tree. Conclusion Cytb gene database of the common rat-shaped animals is created in Jiangsu, in order to lay the foundation for molecular space identification of partial rat-shaped animals.

关 键 词：鼠形动物 CYTB基因 分析 

分 类 号：S443[农业科学—农业昆虫与害虫防治]