Photolyses of mammalian carboxy-hemoglobin studied by photoacoustic calorimetry  

作　　者：ZHAO JinYu LI JiaHuang ZHANG Zheng ZHANG ShuYi QU Min FANG JianWen HUA ZiChun 

机构地区：[1]Laboratory of Modern Acoustics,College of Physics,Nanjing University [2]Laboratory of Pharmaceutical Biotechnology,College of Life Sciences,Nanjing University [3]Nanjing First Hospital attached to Nanjing Medical University [4]Department of Physics,Zhejiang Normal University

出　　处：《Science China(Physics,Mechanics & Astronomy)》2013年第7期1301-1309,共9页中国科学：物理学、力学、天文学（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No. 11074125);the National Basic Research Program of China(Grant No. 2012CB921504);the Foundamental Research Founds for Central Universities of China(Grant No. 1101020402);PAPD Project of Jiangsu Higher Education Institutions

摘　　要：The enthalpy and conformational volume changes in the photolyses of carboxy-hemoglobin (HbCO) of human, bovine, pig, horse and rabbit are investigated by photoacoustic calorimetry. Considering the time scales of the exciting laser pulse and the receiving ultrasound transducers (PVDF films and PZT ceramics), as well as the reaction lifetimes in the photolysis processes of HbCO, the measured results are related to the geminate recombination and tertiary relaxation in photolyses of HbCO. Moreover, the quantum yields of the five mammals are also measured by laser pump-probe technique. The results show that the dynamic parameters, such as enthalpy and conformational volume changes, differ between the processes of the geminate recombination and tertiary relaxation. Also, the dynamic parameters differ among the five mammals although some of them may be consistent with each other.The enthalpy and conformational volume changes in the photolyses of carboxy-hemoglobin （HbCO） of human, bovine, pig, horse and rabbit are investigated by photoacoustic calorimetry. Considering the time scales of the exciting laser pulse and the receiving ultrasound transducers （PVDF films and PZT ceramics）, as well as the reaction lifetimes in the photolysis processes of HbCO, the measured results are related to the geminate recombination and tertiary relaxation in photolyses of HbCO. Moreover, the quantum yields of the five mammals are also measured by laser pump-probe technique. The results show that the dynamic parameters, such as enthalpy and conformational volume changes, differ between the processes of the geminate recombination and tertiary relaxation. Also, the dynamic parameters differ among the five mammals although some of them may be consistent with each other.

关 键 词：photoacoustic calorimetry PHOTOLYSIS enthalpy change conformational volume change carboxy-hemoglobin 

分 类 号：Q51[生物学—生物化学]