水稻穗颈极度缩短突变体sui(t)的遗传分析与基因定位  

Genetic Analysis and Gene Mapping of Extremely Shortened Uppermost Internode Mutant sui(t) in Rice

作　　者：王晓雯[1] 苗润隆 蒋钰东 杜川[1] 杨正林[1] 凌英华[1] 何光华[1] 桑贤春[1] 

机构地区：[1]南方山地农业教育部工程研究中心,西南大学农学与生物科技学院,重庆400716

出　　处：《西南大学学报（自然科学版）》2013年第5期1-6,共6页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31071072;31171178);中央高校基本科研业务费资助项目(XDJK2011c016)

摘　　要：来源于EMS处理籼型恢复系缙恢10号的穗颈节间极度缩短突变体其他节间与恢复系缙恢10号无显著变化,表现为全包穗,对GA3不敏感,暂命名为sui(t).遗传分析表明变异性状受一对隐性核基因控制.以中花11/sui(t)的F2隐性单株为定位群体,将sui(t)基因定位在第1染色体SSR标记RM5336,RM3425向短臂末端一侧,进一步合成SSR,Indel等标记,最终将sui(t)基因定位在RM3148和C1In002(swu)之间,遗传距离分别为0.8cM和1.4cM,物理距离为382kb,为下一步基因克隆和功能研究奠定了基础.One novel mutant, tentatively designated as sui（t）, was discovered from the progeny of indica rice restorer line Jinhuil0, whose seeds were treated with EMS （ethyl methane sulfonate）. This mutant was characterized by fully sheathed panicle and was insensitive to GA3. Morphology analysis showed that its uppermost internode was greatly shortened while the other internodes were normal. Genetic analysis suggested that the mutational character was controlled byasingle recessive gene. Zhonghua11 was crossed with sui（t） and the F2 population was then used for gene mapping. SUI（t） gene was mapped between the SSR markers RM5336/RM3425 and the tag end of the short arm of Chromosome I. SSRandIndelmarkers were designed and SUI（t） gene was finally restricted within the region of RM3148 and ClIn002（swu）, with a genetic distance of 0.8 cM and 1.4 cM, respectively, and a physical distance of 382 kb. The above result provides a foundation for SUI（t） gene cloning and functional analysis.

关 键 词：水稻 顶节间极度缩短 全包穗 GA3不敏感 基因定位 

分 类 号：S511[农业科学—作物学]