曙红Y褪色光度法测定盐酸普罗帕酮  

Fading Spectrophotometric Method for the Determination of Propafenone Hydrochloride with Eosin Y

作　　者：曾岗[1,2] 樊小林[1] 王亚琼[1] 朱经辉[1] 乔曼[1] 胡小莉[1] 

机构地区：[1]西南大学化学化工学院,重庆400715 [2]重庆三峡医药高等专科学校,重庆万州404120

出　　处：《西南大学学报（自然科学版）》2013年第5期107-110,共4页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(20875078)

摘　　要：在pH=3.2的HCl-NaAc缓冲溶液中,曙红Y(EY)与盐酸普罗帕酮(PPF)形成1∶1的离子缔合物,导致溶液的吸收光谱变化,在518nm处产生明显的褪色作用,并在550nm处出现新的吸收峰.PPF的质量浓度在0.2～4.0μg/mL范围内与褪色波长处的吸光度变化值呈良好的线性关系,据此建立一种测定PPF的分光光度新方法.方法简便快捷,灵敏度较高,摩尔吸光系数(ε)为4.16×104 L/(mol.cm).研究了适宜的反应条件和影响因素,并考察了共存物质的影响,表明方法具有较好的选择性,可用于PPF片剂含量测定.In a pH 3.2 HC1-NaAc buffer solution, eosin Y reacted with propafenone hydrochlorid （PPF） to form a 1 ： 1 ion-association complex. As a result, the absorption spectra of solution changed, and an obvious fading peak at 518 nm and a coloring revealing peak at 550 nm appeared. In the range of 0.2-4.0 μg/mL, the concentration of PPF was linearly correlated with the absorbency of the solution at the fading peak, based on which a new sensitive, simple and rapid spectrophotometry method for the determination of PPF was devel oped, the molar absorptivity （e） being 4.16 × 104 L/mol cm. The optimum reaction conditions, the influ encing factors and the effects of coexisting substances were discussed. Applied to the determination of PPF in propafenone hydrochloride tablets, this method gave satisfactory results.

关 键 词：盐酸普罗帕酮 曙红Y 分光光度法 

分 类 号：O657.3[理学—分析化学]