RAPD标记构建家蚕分子连锁图  

Construction of Silkworm RAPD Molecular Linkage Map

作　　者：李斌[1] 鲁成[1] 周泽扬[1] 向仲怀[1] 

机构地区：[1]西南农业大学蚕桑学农业部重点实验室,重庆400716

出　　处：《Acta Genetica Sinica》2000年第2期127-132,共6页

基　　金：国家自然科学基金!39730370;国家教委跨世纪优秀青年基金

摘　　要：以大造、Ｃ１０８及其Ｆ２群体构建了１个家蚕的ＲＡＰＤ连锁框架图，该图含ＲＡＰＤ标记位点１８２个，来自大造的１０３个标记分属前２３个连锁群，来自Ｃ１０８的７９个标记分属后１６个连锁群，覆盖基因组的总长度为１１４８．３ｃＭ（ｃｅｎｔｉｍｏｒｇａｎ），它能与本室用同一群体构建的ＳＡＤＦ图谱相整合，亦可与相应的ＲＦＬＰ图谱相互补充。In this research, a RAPD linkage map of Bombyx mori was constructed with Dazao / C108 and their F2 generation. The map consists of 182 RAPD loci, of which 103 loci come from Dazao and form the first 23 linkage groups and the other 79 loci come from C108 and form the second 16 linkage groups. This map covered a total genetic distance of over 1 148.3 cM (centimorgan). It could be integrated with the SADF map of the same materials constructed in our laboratory and the corresponding RFLP linkage map.

关 键 词：家蚕 分子连锁图 RAPD标记 遗传育种 

分 类 号：S881.24[农业科学—特种经济动物饲养]