羟基红花黄素A对血管内皮细胞黏附分子的影响  

Effects of hydroxy safflor yellow A on vascular cell adhesion molecules

作　　者：吴建良[1] 汪培欢[1] 王翔[1] 杨水新[1] 姚冲[1] 

机构地区：[1]湖州市中心医院,浙江湖州313000

出　　处：《岭南心血管病杂志》2013年第3期352-355,共4页South China Journal of Cardiovascular Diseases

基　　金：湖州市科技计划(项目编号:2011YS09)

摘　　要：目的考察羟基红花黄素A对氧化损伤血管内皮细胞表达黏附分子[血管内皮细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)和细胞间黏附分子-1(intercellular cell adhesion molecule-1,ICAM-1)]的影响。方法用MTS法测定不同浓度羟基红花黄素A对氧化损伤血管内皮细胞与U937细胞黏附率的影响,用反转录聚合酶链反应(RT-PCR)、酶联免疫吸附试验(ELISA)和Western法检测血管内皮细胞黏附分子的表达。结果羟基红花黄素A可以减轻血管内皮细胞与U937细胞的黏附,反转录聚合酶链反应、酶联免疫吸附试验和Western分析结果表明羟基红花黄素A减轻VCAM-1和ICAM-1的表达。结论羟基红花黄素A可通过减轻黏附分子ICAM-1,VCAM-1的表达,抑制白细胞与血管内皮细胞之间的黏附,从而预防氧化对内皮细胞的损伤。Objectives To research the effects of hydroxy safflor yellow A on vascular cell adhesion molecules. Methods Adhesion effects of hydroxy safflor yellow A on umbilical vein endothelial cells was investigated by MTS assay. Expressions of intercellular cell adhesion molecule- 1 （ICAM- 1 ）, vascular cell adhesion molecule- 1 （ VCAM- 1 ） were detected by enzyme linked immunosorbent assay （ELISA）, reverse transcription polymerase chain reaction （RT- PCR） and Western blotting. Results Hydroxy safflor yellow A could inhibit adhesion between endothelial cells and U937 cells. Hydroxy safflor yellow A could inhibit expressions of ICAM-1 and VCAM-1 at gene and protein levels. Conclusions Hydroxy safflor yellow A could inhibit adhesion between endothelial and leucocyte cells by inhibiting expressions of adhesion molecules ICAM-1 and VCAM-1, thus preventing vein endothelial cells from oxidative damage.

关 键 词：羟基红花黄素A 血管内皮细胞黏附分子-1 细胞间黏附分子-1 内皮细胞 氧化 

分 类 号：R331[医药卫生—人体生理学]