用PCR及RFLP方法分析支原体与植原体的同源性  

A homologous comparison between mycoplasma and phytoplasma using 16S rDNA PCR am plification and RFLP analysis

作　　者：邓兆群[1] 胡勤学[2] 柳晟[2] 王瑶[2] 林木兰[2] 

机构地区：[1]湖北医科大学微生物及免疫学教研室,武汉430071 [2]中国科学院武汉病毒研究所,武汉430071

出　　处：《生物多样性》2000年第1期103-105,共3页Biodiversity Science

基　　金：1995年中国科学院生物区系分类资助课题!(960211)

摘　　要：将猪鼻支原体和泡桐丛枝病植原体的16S rDNA进行PCR扩增，分别得到一条1 kb左右的扩增片段。PCR扩增产物用限制性内切酶EcoRⅠ、HindⅢ、BamHⅠ、SalⅠ和SmaⅠ进行RFLP(限制性片段长度多态性)分析，发现用RFLP分析猪鼻支原体和泡桐丛枝病植原体16S rDNA序列同源性的相关系数为072。16S rDNA from mycoplasma and phytoplasma was amplified by PCR, and a band was de tected at about 1 kb. DNA products were digested with restriction enzymes Eco RⅠ,HindⅢ,BamHⅠ,SalⅠ and SmaⅠ, and then were analyzed usi ng RFLP. Similarity coefficient derived from RFLP analysis of mycoplasma and phy toplasma is 0.72.

关 键 词：支原体 植原体 PCR RFLP 同源性分析 亲缘关系 

分 类 号：Q503[生物学—生物化学]