熊果苷对A375与HACAT共培养模型中黑素细胞酪氨酸酶活性的影响  

Effect of Arbutin on the in vitro model of the melanoma cells and keratinocyte co-culture system

作　　者：陈凤江[1] 郭云辉[1] 陈巧云[1] 

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《四川医学》2013年第4期445-447,共3页Sichuan Medical Journal

基　　金：黑龙江省自然科学基金面上项目(编号:D200810)

摘　　要：目的体外构建黑素瘤细胞(A375)与人永生角质形成细胞(HACAT)直接接触共培养模型,观察熊果苷对该模型酪氨酸酶活性的影响同时观察熊果苷对该模型黑素合成及细胞的增殖的影响。方法分别培养A375与HACAT,体外构建黑素瘤细胞与人永生角质形成细胞直接接触的共培养模型;将不同浓度熊果苷作用于此模型,用MTT法、多巴氧化法及NaOH裂解法检测酪氨酸酶活性、黑素合成以及细胞增殖的。结果 A375与HACAT能共同存活;分别用1mg/ml,0.5mg/ml和0.2mg/ml的熊果苷对共培养细胞的增殖、黑素细胞酪氨酸酶活性及黑素合成均有较强的剂量相关的抑制作用,与对照组相比差异有统计学意义(P<0.05或P<0.01)。结论熊果苷对A375与HACAT共培养模型中细胞增殖、酪氨酸酶活性及黑素合成均呈浓度依赖性抑制作用。Objective To establish the in vitro co-culture model of melanoma cells and keratinocytes, and observe the effect of Arbutin on pigmentation in co-culture model of melanoma cells and keratinocytes. Methods We cultivated the melanoma cells and keratinocytes respectively and then constructed the mixed cultivating model of them. After arbutin was added to the model,celludar proliferation , tyrosinase activity and melanin content were measured by MTT assay, L-DOPA oxidation assay and NaOH assay respectively. Results The in vitro co-culture model of melanoma cells and keratinocytes was established successfully The proliferation of co-culture cells, tyrosinase activity and the melanin synthesis were markedly suppressed by arbutin in a dose-dependent manner. The significant suppression was observed with 1,0.5,0. 2mg/ml of arbutin than that with control. Conclusion We have successfully constructed the co-culture model of melanoma cells and keratinocytes,and the results indicate that Arbutin had a strong inhibition on celludar proliferation,tyrosinase activity and melanin content in a dose-dependent manner.

关 键 词：共培养模型 黑素瘤细胞 角质形成细胞 熊果苷 

分 类 号：R285[医药卫生—中药学]