嗜水气单胞菌铁载体的提纯及特性分析  

PURIFICATION AND IDENTIFICATION OF SIDEROPHORE FROM AEROMONAS HYDROPHILA

作　　者：马向东[1] 陆承平[1] 陈怀青[1] 凌红丽[1] 

机构地区：[1]南京农业大学动物医学院

出　　处：《微生物学报》2000年第1期91-94,共4页Acta Microbiologica Sinica

基　　金：国家"九五"攻关项目

摘　　要：提纯了嗜水气单胞菌（Ａｈ）Ｊ１ 株的铁载体（ｓｉｄｅｒｏｐｈｏｒｅ） ，并对其特性进行了初步分析。Ａｈ Ｊ１ 株的培养上清液经聚酰胺柱层析、双蒸水洗脱、乙酸乙脂沉淀和真空冻干，获得白色粉末。用ＣＡＳ法及Ａｒｎｏｗ 法检测均为阳性，证实为铁载体，含有２ ，３二羟基苯甲酸（２ ，３ＤＨＢ） 功能团，属酚盐类铁载体。高压液相色谱分析表明，此种铁载体仅含甘氨酸、赖氨酸及色氨酸。上述纯化的铁载体，在体外培养条件下能促进产铁载体为弱阳性的Ａｈ Ｎ９ａ 株的生长，且能对抗ＥＤＤＡ 对细菌生长的抑制作用，显示铁载体能促进细菌的增殖，在细菌的感染致病过程中可能起重要作用。Siderophore of Aeromonas hydrophila (Ah)J 1 was purified from culture supernatant by polyamide column chromatography,eluted by double\|distilled water,ethyl acetate precipitated,lyophilized,then the white powder was collected,and demonstrated by CAS and Arnow assay.The siderophore was phenolate,composed of 2,3 dihydroxybenzoic acid (2,3 DHB),lysine,glycine and tryptophan by HPLC.Being cultured iniron\|deficient medium,the purified siderophore could stimulated the growth of Ah N9a which siderophore production is poorly positive and reverse the inhibition growth of EDDA.It suggested that siderophore could promote growth of bacteria and might play an important role in bacteria infection.

关 键 词：嗜水气单胞菌 铁载体 纯化 生物学特性 

分 类 号：Q936[生物学—微生物学]