应急大鼠肾上腺髓质嗜铬细胞颗粒数目与其钙含量的关系  

Neural control of the compensatory increase of testosterone in hemicastrated adult male rats 

作　　者：刘懿[1] 凌诒萍[1] 俞彰[1] 吴正泉[1] 钟慈声[1] 

机构地区：[1]上海医科大学生物物理学教研室,上海200032

出　　处：《生理学报》2000年第1期5-9,共5页Acta Physiologica Sinica

摘　　要：采用电镜细胞立体形态计量法及电镜X射线显微定量分析术 ,对制动应急大鼠的肾上腺髓质细胞内嗜铬颗粒数密度和颗粒内Ca浓度变化进行测量。结果显示 ,两者在制动过程中均呈进行性下降 ,但颗粒内钙浓度的下降快于颗粒数目的减少 (P <0 0 1)。该结果支持颗粒内钙释放入胞质 ,参与胞质内游离钙浓度的升高 ,进而激发颗粒胞吐的假设 。It is unclear why the concentration of testosterone increases in the testicular vein after hemicastration without cor^responding alteration in gonadotropins. The present work was undertaken to examine whether the testosterone levels could be modified by denervation of the testis in adult rats. Both systemic and testicular blood samples were collected either immediately before or 6 and 24 hours after hemicastration from the rats two weeks after denervation of either inferior spermatic nerves (ISN) or ISN plus superior spermatic nerves (ISN SSN). Increase of testicular testosterone induced by hemicastration was significantly ( P <0 05) inhibited in these rats, as compared with the sham animals (at 6 and 24 hours, ISN vs sham: 16 00±3 35 vs 42 72±13 85 and 26 93±8 68 vs 71 16±13 30 whilst ISN SSN vs sham: 31 63±7 92 vs 60 61±18 11 and 27 70±8 93 vs 93 92±19 73 ng/ml, respectively), whereas no significant change in LH was observed in all the experimental groups. FSH underwent no alteration in all the ISN denervation groups, but a significant elevation was observed in the ISN SSN denervation groups ( P <0 05) before hemicastration. Therefore, it appears that the change in FSH is not the cause of the inhibition of testosterone increase in the hemicastrated rats after testicular denervation and that ISN plays an active role in regulation of testosterone increase induced by hemicastration.

关 键 词：肾上腺髓质细胞 嗜铬颗粒 钙含量 细胞内钙库 

分 类 号：Q454[生物学—生理学]